FOSFADECIN AND FOSFOCYTOCIN, NEW NUCLEOTIDE ANTIBIOTICS PRODUCED BY BACTERIA

• Published in: Journal of antibiotics 1990/03/25, Vol.43(3), pp.238-246
• Main Authors: KATAYAMA, NOZOMI, TSUBOTANI, SHIGETOSHI, NOZAKI, YUKIMASA, HARADA, SETSUO, ONO, HIDEO
• Format: Article
• Language: English
• Subjects: Adenosine Diphosphate - analogs & derivatives; Adenosine Diphosphate - analysis; Adenosine Diphosphate - isolation & purification; Adenosine Diphosphate - pharmacology; Adenosine Diphosphate - therapeutic use; Animals; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibiotics (antibacterial agents, antifungal agents); Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents; Applied microbiology; Bacteria - drug effects; Biological and medical sciences; Chromatography; Cytidine Diphosphate - analogs & derivatives; Cytidine Diphosphate - analysis; Cytidine Diphosphate - isolation & purification; Cytidine Diphosphate - pharmacology; Cytidine Monophosphate - analogs & derivatives; Cytidine Monophosphate - analysis; Cytidine Monophosphate - isolation & purification; Cytidine Monophosphate - pharmacology; Escherichia coli Infections - drug therapy; Fermentation; fosfadecin; fosfocytocin; Fosfomycin - analysis; Fosfomycin - isolation & purification; Fosfomycin - pharmacology; Fundamental and applied biological sciences. Psychology; gram-negative bacteria; gram-positive bacteria; Hydrolysis; Magnetic Resonance Spectroscopy; Mice; Microbiology; Molecular Structure; Pseudomonas - classification; Pseudomonas - metabolism; Pseudomonas fluorescens; Staphylococcal Infections - drug therapy
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.43.238

Two new nucleotide antibiotics, fosfadecin and fosfocytocin, have been isolated from the culture filtrates of Pseudomonas viridiflava PK-5 and Pseudomonas fluorescens PK-52, respectively. These antibiotics were purified by column chromatographies using adsorption, gel filtration and ion exchange resins. On the basis of the spectroscopic and degradation studies, the chemical structures of fosfadecin and fosfocytocin were determined. These antibiotics were either enzymatically or chemically hydrolyzed to generate fosfomycin and a new antibiotic, fosfoxacin, which are also produced in the culture filtrates. They showed antibacterial activity against Gram-positive and Gram-negative bacteria. The antibacterial activity of these nucleotide antibiotics was weaker than that of fosfomycin and fosfoxacin.