In vitro enhancement of T-cell receptor-targeted lysis after IL-2 treatment of PBL from HIV-seropositive individuals

Individuals with advanced HIV infection have a greater proportion of T-cells that are activated when compared to uninfected individuals. These activated cells are not able to lyse specific targets. The reason for this dysfunction is not well known. In this study we demonstrate that there are CD8+ T-...

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Bibliographic Details
Published in:Clinical immunology and immunopathology 1998-02, Vol.86 (2), p.226-234
Main Authors: KOLBER, M. A, HURLEY, J, MORENO, J. N, CABRAL, L
Format: Article
Language:English
Subjects:
Adult
AIDS/HIV
Animals
Biological and medical sciences
CD3 Complex - immunology
CD8-Positive T-Lymphocytes - immunology
Cytotoxicity, Immunologic
Female
HIV Seropositivity - immunology
Humans
Immunomodulators
Interleukin-2 - pharmacology
Male
Medical sciences
Mice
Mice, Inbred DBA
Middle Aged
Pharmacology. Drug treatments
Receptors, Antigen, T-Cell, alpha-beta - immunology
Tumor Cells, Cultured
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Summary:Individuals with advanced HIV infection have a greater proportion of T-cells that are activated when compared to uninfected individuals. These activated cells are not able to lyse specific targets. The reason for this dysfunction is not well known. In this study we demonstrate that there are CD8+ T-cells from HIV-seropositive individuals that can be targeted to lyse targets with OKT3 (anti-CD3 antibody) but are unable to lyse targets with WT31 (anti-alphabeta antibody). Treatment of peripheral blood lymphocytes with IL-2 results in an enhancement of WT31-targeted lysis in 9 of 13 individuals evaluated. These findings demonstrate a differential response, in vitro, of CD8+ T-cells to IL-2 treatment. Future work evaluating clinical responses after IL-2 therapy with recovery of targeted lysis in vitro could provide information on screening of individuals for therapeutic intervention.
ISSN:0090-1229
1090-2341
DOI:10.1006/clin.1997.4474