Crosstalk: phosphorylation of alpha1b-adrenoceptors induced through activation of bradykinin B2 receptors

The action of bradykinin was studied in rat-1 fibroblasts stably expressing alpha1b-adrenoceptors. It was observed that bradykinin and kallidin markedly increase cytosol calcium concentration, but that the B1 agonist, des-Arg9-bradykinin, only mimicked this effect to a minimal extent. Antagonists, s...

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Bibliographic Details
Published in:FEBS letters 1998-01, Vol.422 (2), p.141-145
Main Authors: Medina, L C, Vázquez-Prado, J, Torres-Padilla, M E, Mendoza-Mendoza, A, Cruz Muñoz, M E, García-Sáinz, J A
Format: Article
Language:English
Subjects:
Animals
Bradykinin - analogs & derivatives
Bradykinin - pharmacology
Calcium - metabolism
Cell Line
Cricetinae
Cytosol - metabolism
Endothelins - pharmacology
Inositol Phosphates - metabolism
Kallidin - pharmacology
Norepinephrine - pharmacology
Phosphorylation
Rats
Receptor, Bradykinin B2
Receptors, Adrenergic, alpha-1 - drug effects
Receptors, Adrenergic, alpha-1 - physiology
Receptors, Bradykinin - drug effects
Receptors, Bradykinin - physiology
Recombinant Proteins - drug effects
Recombinant Proteins - metabolism
Signal Transduction
Transfection
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Summary:The action of bradykinin was studied in rat-1 fibroblasts stably expressing alpha1b-adrenoceptors. It was observed that bradykinin and kallidin markedly increase cytosol calcium concentration, but that the B1 agonist, des-Arg9-bradykinin, only mimicked this effect to a minimal extent. Antagonists, selective for the B2 subtype, such as Hoe 140, blocked this effect of bradykinin and kallidin. Similarly, bradykinin and kallidin stimulated the production of inositol phosphates and B2 antagonists blocked their actions. The possibility that bradykinin could modulate alpha1b-adrenoceptors was studied. It was observed that bradykinin and kallidin increased alpha1b-adrenoceptor phosphorylation and that such effect was also blocked by Hoe 140. Interestingly, the ability of norepinephrine to increase intracellular calcium concentration was not altered by pretreatment of the cells with bradykinin, i.e. bradykinin induced alpha1b-adrenoceptor phosphorylation but this did not lead to receptor desensitization.
ISSN:0014-5793