Molecular cloning and sequencing of a chitin synthase gene (CHS2) of Paracoccidioides brasiliensis

The nucleotide sequence of a chitin synthase gene (CHS2) of the dimorphic fungal human pathogen Paracoccidioides brasiliensis has been determined. The deduced amino acid sequence of Chs2p consists of 1043 residues and is highly homologous to other class II fungal chitin synthases. Computational stru...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 1998-01, Vol.14 (2), p.181-187
Main Authors: Niño‐Vega, Gustavo A., Buurman, Ed T., Gooday, Graham W., San‐Blas, Gioconda, Gow, Neil A. R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
chitin synthase
Chitin Synthase - chemistry
Chitin Synthase - genetics
Cloning, Molecular
dimorphic fungi
Molecular Sequence Data
Paracoccidioides - enzymology
Paracoccidioides - genetics
Paracoccidioides brasiliensis
Polymerase Chain Reaction
Sequence Analysis, DNA
Sequence Homology, Amino Acid
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Summary:The nucleotide sequence of a chitin synthase gene (CHS2) of the dimorphic fungal human pathogen Paracoccidioides brasiliensis has been determined. The deduced amino acid sequence of Chs2p consists of 1043 residues and is highly homologous to other class II fungal chitin synthases. Computational structural analyses suggest very high similarity to other fungal chitin synthases with a highly variable region at the cytosolic amino‐terminal region which may be related to its possible zymogenic nature, and the putative catalytic region close to seven membrane‐spanning regions at the carboxyl terminus. The nucleotide sequence of CHS2 and its flanking regions has been submitted to GenBank under Accession Number Y09231. © 1998 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/(SICI)1097-0061(19980130)14:2<181::AID-YEA202>3.0.CO;2-D