Protective roles of two aluminum (Al)-induced genes, HSP150 and SED1 of Saccharomyces cerevisiae, in Al and oxidative stresses

Abstract We isolated two yeast cDNA clones whose transcripts are induced by aluminum (Al) metal stress. Partial nucleotide sequencing showed that one is the HSP150 gene encoding a secreted heat shock protein, and the other corresponds to the SED1 gene encoding a putative membrane protein. To clarify...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1998-02, Vol.159 (1), p.99-105
Main Authors: Ezaki, Bunichi, Gardner, Richard C, Ezaki, Yuka, Kondo, Hideki, Matsumoto, Hideaki
Format: Article
Language:English
Subjects:
Action of physical and chemical agents
Aluminum
Aluminum - pharmacology
Aluminum metal stress
Biological and medical sciences
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
Fungal Proteins - physiology
Gene Expression Regulation, Fungal - drug effects
Gene sequencing
Genes
Genes, Fungal - physiology
Glycoproteins
Heat shock proteins
Heat shock stress
Heat-Shock Proteins - physiology
Heat-Shock Response - genetics
HSP150 gene
Hsp150 protein
Membrane Glycoproteins - physiology
Membrane proteins
Microbiology
Mutation
Mycology
Nucleotides
Oxidative stress
Oxidative Stress - genetics
Phenotype
Proteins
RNA, Fungal - analysis
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
SED1 gene
Sensitivity analysis
Yeast
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract We isolated two yeast cDNA clones whose transcripts are induced by aluminum (Al) metal stress. Partial nucleotide sequencing showed that one is the HSP150 gene encoding a secreted heat shock protein, and the other corresponds to the SED1 gene encoding a putative membrane protein. To clarify the biological functions of these genes, we analyzed the sensitivity of gene-disrupted mutants to Al stress and to oxidative stresses. The Al tests indicated that the HSP150 protein served a basal protective role in Al stress, but SED1 did not; both of the genes had protective roles for oxidative stresses. The results for the HSP150 gene suggest that there is an overlap between Al ion stress, oxidative stress and heat shock stress in yeast.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1998.tb12847.x