Demonstration of a Surface Antigen of Clostridium tyrobutyricum by Use of Immunoblotting with a Monoclonal Antibody

A monoclonal antibody, prepared against whole cells of Clostridium tyrobutyricum, recognized a surface antigen extracted by heat treatment or by hot phenol-water treatment. This antigen, after analysis by polyacrylamide gel electrophoresis and immunoblotting, has been shown to present a regularly-sp...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1990, Vol.34(1), pp.55-64
Main Authors: Gueguen, Françoise, Robreau, Georges, Talbot, Françoise, Malcoste, Roger
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Antigens, Bacterial - analysis
Antigens, Surface - analysis
Bacteriology
Biological and medical sciences
Blotting, Western - methods
Cell Wall - immunology
Clostridium - immunology
Clostridium tyrobutyricum
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Hot Temperature
Microbiology
Morphology, structure, chemical composition
Phenols
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Description
Summary:A monoclonal antibody, prepared against whole cells of Clostridium tyrobutyricum, recognized a surface antigen extracted by heat treatment or by hot phenol-water treatment. This antigen, after analysis by polyacrylamide gel electrophoresis and immunoblotting, has been shown to present a regularly-spaced ladder pattern similar to those shown by the lipopolysaccharide of many gram-negative bacteria. The proteinase K has been shown to have no effect on the recognition of this epitope by the monoclonal antibody. On the contrary, the inhibition of the antigen reactivity to the monoclonal antibody after a mild periodate oxidation suggests the involvement of a carbohydrate moiety in the epitope. Moreover, the SDS-PAGE analysis of phenol-water extracts has shown an additional compound, detected by silver staining but not recognized by the monoclonal antibody.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1990.tb00991.x