Quantitative Determination of Stanozolol and Its Metabolite in Urine by Gas Chromatography/Mass Spectrometry

The amount of stanozolol and 3′-hydroxystanozolol in urine was determined after oral administration of stanozolol. A gas chromatograph/mass selective detector equipped with a capillary column was used for these determinations. The GC/MS was operated in the SIM mode, and m/z 581, 669, and 315 were mo...

Full description

Saved in:
Bibliographic Details
Published in:Journal of analytical toxicology 1990-03, Vol.14 (2), p.109-112
Main Authors: Choo, Hea-Young P., Kwon, Oh-Seung, Park, Jongsei
Format: Article
Language:English
Subjects:
Administration, Oral
Analysis
Biological and medical sciences
Doping in Sports
Excretion
Gas chromatography
Gas Chromatography-Mass Spectrometry
General pharmacology
Humans
Male
Mass spectroscopy
Medical sciences
Metabolites
Oral administration
Pharmacology. Drug treatments
stanozolol
Stanozolol - administration & dosage
Stanozolol - urine
Steroid hormones
Substance Abuse Detection - methods
Time Factors
Urine
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The amount of stanozolol and 3′-hydroxystanozolol in urine was determined after oral administration of stanozolol. A gas chromatograph/mass selective detector equipped with a capillary column was used for these determinations. The GC/MS was operated in the SIM mode, and m/z 581, 669, and 315 were monitored for stanozolol, 3′-hydroxystanozolol, and calusterone (internal standard), respectively. The detection limit was approximately 1 ng/mL for both steroids. The maximum excretion rate of stanozolol was reached in 8 h and the maximum of 3′-hydroxystanozolol in 19 h. However, only 3% of the administered amount was recovered in urine.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/14.2.109