Studies on in vitro effect of free fatty acids on water content and osmotic fragility of rabbit (lepus cuniculus) erythrocytes

The effects of free fatty acids in the blood on osmotic fragility and water content of erythrocytes when combined with hyperthermia were investigated. The isotonic buffer, containing the fatty acid, was added to the erythrocyte suspension to a final concentration of 200μM/L. The samples were kept fo...

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Bibliographic Details
Published in:Life sciences (1973) 1998, Vol.62 (9), p.823-828
Main Authors: Kogawa, Hiroshi, Yabushita, Noriko, Nakajima, Toshifumi, Kageyama, Katsuhiro
Format: Article
Language:English
Subjects:
Animals
Chromatography, Gas
erythrocytes
Erythrocytes - drug effects
Erythrocytes - metabolism
Fatty Acids, Nonesterified - pharmacology
Hot Temperature
Humans
hyperthermia
In Vitro Techniques
Intracellular Fluid
intracellular water content
Osmotic Fragility
Rabbits
sports anemia
unsaturated fatty acid
Water
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Summary:The effects of free fatty acids in the blood on osmotic fragility and water content of erythrocytes when combined with hyperthermia were investigated. The isotonic buffer, containing the fatty acid, was added to the erythrocyte suspension to a final concentration of 200μM/L. The samples were kept for one hour in an incubator at 37 °C or 42 °C. The osmotic fragility of erythrocytes was determined by coil planet centrifuge system and intracellular water content was measured by gasliquid chromatography. A high concentration of unsaturated fatty acid and hyperthermia caused the increase in intracellular water and the decrease in osmotic resistance of the red blood cells compared to saturated fatty acid and the control. The present experiment demonstrated that unsaturated fatty acid was one of the principal chemical and metabolic factors which caused sports anemia.
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(97)01184-3