Strategies for selection of antibodies by phage display

Phage antibody-display is rapidly maturing into a very effective tool for antibody generation. The recent development of large primary antibody libraries enables selection of antibodies against most targets in under two weeks and many of these antibodies have relatively high (nanomolar) affinities....

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Bibliographic Details
Published in:Current opinion in biotechnology 1998-02, Vol.9 (1), p.102-108
Main Authors: Griffiths, Andrew D, Duncan, Alexander R
Format: Article
Language:English
Subjects:
Animals
Antibodies - genetics
Antibodies - immunology
Antibody Affinity
Bacteriophages - genetics
Biotechnology - methods
Gene Library
Genetic Vectors
Humans
Immunization
Immunoglobulin Variable Region - genetics
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Summary:Phage antibody-display is rapidly maturing into a very effective tool for antibody generation. The recent development of large primary antibody libraries enables selection of antibodies against most targets in under two weeks and many of these antibodies have relatively high (nanomolar) affinities. Successful strategies have also been developed to affinity mature these antibodies into the picomolar range if required.
ISSN:0958-1669
1879-0429
DOI:10.1016/S0958-1669(98)80092-X