In vivo induction of functional Fc gammaRI (CD64) on neutrophils and modulation of blood cytokine mRNA levels in cancer patients treated with G-CSF (rMetHuG-CSF)

Neutrophils from 13 children who received G-CSF for the collection of peripheral blood progenitors while they were in haematological steady state were studied at various times after G-CSF injection for Fc gammaR expression (Fc gammaRI or CD64, Fc gammaRII or CD32, and Fc gammaRIII or CD16) and for t...

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Bibliographic Details
Published in:British journal of haematology 1998-03, Vol.100 (3), p.550-556
Main Authors: Michon, J M, Gey, A, Moutel, S, Tartour, E, Meresse, V, Fridman, W, Teillaud, J L
Format: Article
Language:English
Subjects:
Adolescent
Antibody-Dependent Cell Cytotoxicity - immunology
Child
Child, Preschool
Cytokines - genetics
Cytokines - metabolism
Female
Filgrastim
Granulocyte Colony-Stimulating Factor - therapeutic use
Hematopoietic Stem Cell Mobilization
Humans
Infant
Interleukin-8 - pharmacology
Leukocyte Count
Male
Neoplasms - drug therapy
Neoplasms - immunology
Neutrophils - immunology
Receptors, IgG - immunology
Recombinant Proteins
RNA, Messenger - metabolism
Up-Regulation
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Summary:Neutrophils from 13 children who received G-CSF for the collection of peripheral blood progenitors while they were in haematological steady state were studied at various times after G-CSF injection for Fc gammaR expression (Fc gammaRI or CD64, Fc gammaRII or CD32, and Fc gammaRIII or CD16) and for their ability to exert antibody-dependent cell cytotoxicity (ADCC) through Fc gammaRI. Changes in IFNgamma, IL8, IL10, MCP1 and TNF alpha mRNA levels in peripheral blood cells were also studied 4 h and 24 h after the first G-CSF injection. Fc gammaRI expression increased strongly after 24 h and then remained at the same level throughout treatment. In contrast, Fc gammaRIII expression sharply decreased at day 1 and diminished even further thereafter. No change in Fc gammaRII was observed. ADCC exerted by neutrophils through Fc gammaRI started to increase after 24 h with the peak level at day 5. Cytokine mRNA analyses indicated a reproducible and strong increase of IL8 mRNA (11/13 children) after 24 h, whereas the changes in the mRNA levels of the other cytokines tested were more heterogenous (TFNgamma: three; IL10: six; MCP1: five: TNF alpha: four, of the 13 children). Therefore this study opens the way to an optimized therapeutic schedule for the combined use of G-CSF and monoclonal antibodies in adjuvant immuno-intervention.
ISSN:0007-1048