Localization of Motor-Related Proteins and Associated Complexes to Active, but Not Inactive, Centromeres

Multicentric chromosomes are often found in tumor cells and certain cell lines. How they are generated is not fully understood, though their stability suggests that they are non-functional during chromosome segregation. Growing evidence has implicated microtubule motor proteins in attachment of chro...

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Bibliographic Details
Published in:Human molecular genetics 1998-04, Vol.7 (4), p.671-677
Main Authors: Faulkner, Nicole E., Vig, Baldev, Echeverri, Christophe J., Wordeman, Linda, Vallee, Richard B.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomarkers - analysis
Centromere - chemistry
Chromosomal Proteins, Non-Histone - analysis
Cytogenetics
Dynactin Complex
Dyneins - analysis
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Human
Humans
Kinesin - analysis
Microfilament Proteins
Microtubule Proteins - analysis
Microtubule-Associated Proteins - analysis
Tumor Cells, Cultured
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Summary:Multicentric chromosomes are often found in tumor cells and certain cell lines. How they are generated is not fully understood, though their stability suggests that they are non-functional during chromosome segregation. Growing evidence has implicated microtubule motor proteins in attachment of chromosomes to the mitotic spindle and in chromosome movement. To better understand the molecular basis for the inactivity of centromeres associated with secondary constrictions, we have tested these structures by immunofluorescence microscopy for the presence of motor complexes and associated proteins. We find strong immunoreactivity at the active, but not inactive, centromeres of prometaphase multicentric chromosomes using antibodies to the cytoplasmic dynein intermediate chains, three components of the dynactin complex (dynamitin, Arp1 and p150Glued), the kinesin-related proteins CENP-E and MCAK and the proposed structural and checkpoint proteins HZW10, CENP-F and Mad2p. These results offer new insight into the assembly and composition of both primary and secondary constrictions and provide a molecular basis for the apparent inactivity of the latter during chromosome segregation.
ISSN:0964-6906
1460-2083
1460-2083
DOI:10.1093/hmg/7.4.671