Increased expression of NADH-ubiquinone oxidoreductase chain 2 (ND2) in preimplantation rabbit embryos cultured with 20% oxygen concentration

In vitro culture of mammalian preimplantation embryos is associated with various developmental disorders such as retardation in development and cell damage. The molecular mechanisms underlying these processes are poorly understood. One of the possible reasons may be the unphysiologically high oxygen...

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Bibliographic Details
Published in:Molecular reproduction and development 1998-04, Vol.49 (4), p.394-399
Main Authors: Koerber, Sabine, Santos, Anne Navarrete, Tetens, Frank, Küchenhoff, Andreas, Fischer, Bernd
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding, Competitive
Biological and medical sciences
Blastocyst - enzymology
Blastocyst - metabolism
Cells, Cultured
Culture Media - pharmacology
Electron Transport Complex I
Female
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Developmental - drug effects
Horses
in vitro culture
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
NADH, NADPH Oxidoreductases - biosynthesis
NADH, NADPH Oxidoreductases - genetics
NADH-ubiquinone oxidoreductase chain 2
oxidative stress
Oxygen - metabolism
Oxygen - pharmacology
Polymerase Chain Reaction
preimplantation embryo
rabbit
Rabbits
RNA, Messenger - biosynthesis
Sequence Homology, Nucleic Acid
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Summary:In vitro culture of mammalian preimplantation embryos is associated with various developmental disorders such as retardation in development and cell damage. The molecular mechanisms underlying these processes are poorly understood. One of the possible reasons may be the unphysiologically high oxygen concentration used for culture. Four‐day‐old rabbit blastocysts were cultured with 5% O2 (physiologic oxygen concentration) or 20% O2 (usually used for in vitro culture) for 4 hr. Differences in gene expression were analysed by differential display reverse‐transcriptase polymerase chain reaction (DD RT‐PCR). Thirty‐two differentially expressed RNA bands were found. Two of them revealed a high sequence homology to the equine NADH‐ubiquinone oxidoreductase chain 2 (ND2), a subunit of complex I of the respiratory chain. mRNA expression of ND2 was increased in blastocysts cultured with the higher oxygen concentration. Increased expression of ND2 was confirmed by semiquantitative and semiquantitative competitive RT‐PCR. Mol. Reprod. Dev. 49:394–399, 1998. © 1998 Wiley‐Liss, Inc.
ISSN:1040-452X
1098-2795
DOI:10.1002/(SICI)1098-2795(199804)49:4<394::AID-MRD6>3.0.CO;2-I