Regulation of Human Eosinophil Precursor Production by Cytokines: A Comparison of Recombinant Human Interleukin-1 (rhIL-1), rhIL-3, rhIL-5, rhIL-6, and rh Granulocyte-Macrophage Colony-Stimulating Factor

The effect of a panel of recombinant human (rh) cytokines on the generation of human eosinophil precursors was assessed using a two-step culture technique. Normal human bone marrow was preincubated with different cytokine combinations in liquid culture before assessment of the number of eosinophil p...

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Bibliographic Details
Published in:Blood 1990-05, Vol.75 (9), p.1774-1779
Main Authors: Clutterbuck, Elaine J., Sanderson, Colin J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Factors - pharmacology
Bone Marrow Cells
Cell physiology
Cells, Cultured
Colony-Forming Units Assay
Cytokines
Eosinophils - cytology
Eosinophils - drug effects
Fundamental and applied biological sciences. Psychology
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - drug effects
Humans
Interleukin-1 - pharmacology
Interleukin-3 - pharmacology
Interleukin-5 - pharmacology
Interleukin-6 - pharmacology
Molecular and cellular biology
Recombinant Proteins - pharmacology
Responses to growth factors, tumor promotors, other factors
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Summary:The effect of a panel of recombinant human (rh) cytokines on the generation of human eosinophil precursors was assessed using a two-step culture technique. Normal human bone marrow was preincubated with different cytokine combinations in liquid culture before assessment of the number of eosinophil progenitors, which give rise to eosinophil colony-forming units (CFU-Eo) on secondary semi-solid culture with either interleukin-5 (IL-5), IL-3, or granulocyte-macrophage colony-stimulating factor. rhIL-3 or rhGM-CSF, but not rhIL-5, increased the number of CFU-Eo. CFU-Eo production by rhIL-3 or rhGMCSF was maximal after 7 days' preincubation. Neither rhIL-1 or rhIL-6 acted on eosinophil precursors, either alone or in combination with rhIL-5, rhIL-3, or rhGM-CSF. A similar spectrum of activity of the cytokines was demonstrated whether rhIL-5, rhIL-3, or rhGM-CSF was used in the secondary cultures as the eosinophil CSF. However, rhIL-3 induced relatively more rhIL-5-responsive CFU-Eo than rhIL-3-responsive CFU-Eo, suggesting that rhIL-3 is pushing progenitors into an rhIL-5-responsive compartment.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V75.9.1774.1774