Apple messenger RNAs related to bacterial lignostilbene dioxygenase and plant SAUR genes are preferentially expressed in flowers

In an attempt to use a differential display procedure to identify organ-specific genes in apple, cDNA fragments of two transcripts preferentially expressed in flowers were isolated and corresponding full-length cDNA inserts were subsequently obtained. One of these clones, Md-FS1, belongs to the SAUR...

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Bibliographic Details
Published in:Plant molecular biology 1998-04, Vol.36 (6), p.909-915
Main Author: Watillon, B. (Faculte Universitaire des Sciences Agronomiques, Gembloux (Belgium). Unite de Biologie Moleculaire et Physiologie Animale)
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Apples
ARN MENSAJERO
ARN MESSAGER
Biodegradation
Blotting, Southern
Dioxygenases
DNA, Complementary - chemistry
DNA, Plant - chemistry
DNA, Plant - metabolism
EXPRESION GENICA
EXPRESSION DES GENES
FLEUR
FLORES
FLOWERS
GENE
GENE EXPRESSION
GENES
Indoleacetic Acids - pharmacology
Isoenzymes - genetics
MALUS PUMILA
MESSENGER RNA
Molecular Sequence Data
Open Reading Frames
Oxygenases - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Shoots - enzymology
Plant Shoots - genetics
Plant Shoots - growth & development
Proteins
RNA, Messenger - metabolism
RNA, Plant - metabolism
Rosales - enzymology
Rosales - genetics
Rosales - growth & development
Sequence Analysis, DNA
Shoots
Soybeans
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Summary:In an attempt to use a differential display procedure to identify organ-specific genes in apple, cDNA fragments of two transcripts preferentially expressed in flowers were isolated and corresponding full-length cDNA inserts were subsequently obtained. One of these clones, Md-FS1, belongs to the SAUR gene family, originally identified as a set of auxin-inducible genes in soybean. The second one, Md-FS2, encodes a polypeptide with sequence similarities to bacterial lignostilbene-alpha,beta-dioxygenase isozymes, which are thought to be involved in lignin biodegradation. Northern blot analysis confirmed that both genes are preferentially expressed in floral organs at full bloom, while being expressed at lower or undetectable levels in vegetative organs (leaves, shoots or roots) as well as in immature, green and unopened blossoms. Furthermore, Md-FS1 transcripts also appeared to accumulate in vegetative tissues after auxin treatment of micropropagated apple shoots.
ISSN:0167-4412
1573-5028
DOI:10.1023/A:1005914506110