Transfer and expression of the tetracycline resistance transposon Tn925 in Acetobacterium woodii

The Enterococcus faecalis conjugative plasmid pCF10 was used to introduce Tn925 into Acetobacterium woodii by filter mating. Tetracycline resistance was transferred at frequencies of about 10(-6) per donor, but no plasmid DNA was found in the transconjugants. DNA hybridization analyses of HindIII-di...

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Bibliographic Details
Published in:FEMS microbiology letters 1990-03, Vol.68 (1-2), p.171-176
Main Authors: STRÄTZ, M, GOTTSCHALK, G, DÜRRE, P
Format: Article
Language:English
Subjects:
antibiotic resistance
Bacillus subtilis - genetics
Bacteriology
Biological and medical sciences
Blotting, Southern
Conjugation, Genetic
DNA
DNA Transposable Elements
DNA, Bacterial - genetics
Enterococcus faecalis - genetics
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
gene expression
gene transfer
Genetics
Gram-Positive Bacteria - genetics
hybridization analysis
Microbiology
Plasmids
tetracycline
Tetracycline Resistance - genetics
transposons
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Summary:The Enterococcus faecalis conjugative plasmid pCF10 was used to introduce Tn925 into Acetobacterium woodii by filter mating. Tetracycline resistance was transferred at frequencies of about 10(-6) per donor, but no plasmid DNA was found in the transconjugants. DNA hybridization analyses of HindIII-digested chromosomal DNA demonstrated the insertion of Tn925 at a variety of locations, whereas wild type DNA showed no hybridization at all. The transconjugants were used as donor in mating experiments with tetracycline-sensitive Bacillus subtilis. Transfer of tetracycline resistance was observed at frequencies of 10(-8) per recipient.
ISSN:0378-1097
1574-6968