Glutamylation of centriole and cytoplasmic tubulin in proliferating non-neuronal cells

We have examined the distribution of glutamylated tubulin in non‐neuronal cell lines. A major part of centriole tubulin is highly modified on both the α‐ and β‐tubulin subunits, whereas a minor part of the cytoplasmic tubulin is slightly modified, on the β‐tubulin only. Furthermore, we observed that...

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Bibliographic Details
Published in:Cell motility and the cytoskeleton 1998, Vol.39 (3), p.223-232
Main Authors: Bobinnec, Y., Moudjou, M., Fouquet, J.P., Desbruyères, E., Eddé, B., Bornens, M.
Format: Article
Language:English
Subjects:
Animals
Cell Line
centriole
Centrioles - chemistry
Cytoplasm - chemistry
glutamylation
Humans
Kinetochores - chemistry
Lymphoid Tissue
Mice
microtubule
Microtubules - chemistry
Mitosis
Polyglutamic Acid - analysis
Spindle Apparatus - chemistry
tubulin
Tubulin - analysis
tubulin post-translational modification
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Summary:We have examined the distribution of glutamylated tubulin in non‐neuronal cell lines. A major part of centriole tubulin is highly modified on both the α‐ and β‐tubulin subunits, whereas a minor part of the cytoplasmic tubulin is slightly modified, on the β‐tubulin only. Furthermore, we observed that tubulin glutamylation varies during the cell cycle: an increase occurs during mitosis on both centriole and spindle microtubules. In the spindle, this increase appears more obvious on the pole‐to‐pole and kinetochore microtubules than on the astral microtubules. The cellular pattern and the temporal variation of this post‐translational modification contrast with other previously described tubulin modifications. The functional significance of this distribution is discussed. Cell Motil. Cytoskeleton 39:223–232, 1998. © 1998 Wiley‐Liss, Inc.
ISSN:0886-1544
1097-0169
DOI:10.1002/(SICI)1097-0169(1998)39:3<223::AID-CM5>3.0.CO;2-5