Collaborative and Reciprocal Effects of Ciliary Neurotrophic Factor and Nerve Growth Factor on the Neuronal Phenotype of Human Neuroblastoma Cells

: We have probed the molecular basis of functional effects of ciliary neurotrophic factor (CNTF) and nerve growth factor (NGF) on aspects of the neuronal differentiation of LA‐N‐2 neuroblastoma cells. The influence of CNTF on the cholinergic phenotype can be accounted for by transcriptional/translat...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neurochemistry 1998-04, Vol.70 (4), p.1411-1420
Main Authors: Zinman, L. H., Lawrance, G., Wang, W., Verge, V. M. K., Dow, K. E., Maurice, D. H., Richardson, P. M., Riopelle, R. J.
Format: Article
Language:English
Subjects:
Biochemistry and metabolism
Biological and medical sciences
Cell Adhesion - drug effects
Central nervous system
Choline O-Acetyltransferase - genetics
Choline O-Acetyltransferase - metabolism
Ciliary Neurotrophic Factor
Fundamental and applied biological sciences. Psychology
Humans
In Situ Hybridization
Nerve growth factor
Nerve Growth Factors - pharmacology
Nerve Growth Factors - physiology
Nerve Tissue Proteins - pharmacology
Neurite growth
Neuroblastoma - genetics
Neuroblastoma - pathology
Neurons - drug effects
Neurons - physiology
Phenotype
Polymerase Chain Reaction
Receptor, Nerve Growth Factor
Receptor, trkA - genetics
Receptor, trkA - metabolism
Receptors, Nerve Growth Factor - genetics
Receptors, Nerve Growth Factor - metabolism
RNA, Messenger - metabolism
Survival
Transcription, Genetic
Trk
Tumor Cells, Cultured
Vertebrates: nervous system and sense organs
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:: We have probed the molecular basis of functional effects of ciliary neurotrophic factor (CNTF) and nerve growth factor (NGF) on aspects of the neuronal differentiation of LA‐N‐2 neuroblastoma cells. The influence of CNTF on the cholinergic phenotype can be accounted for by transcriptional/translational effects without implicating posttranslational mechanisms. Although both NGF receptors are expressed constitutively by LA‐N‐2 cells, CNTF has a marked stimulatory effect on trkA mRNA and protein. The NGF receptors are functional in serum‐free conditions where they mitigate CNTF effects on cell adhesion but do not support process extension. Following priming by CNTF, NGF and CNTF have synergistic influences on process formation but not on choline acetyltransferase‐specific activity.
ISSN:0022-3042
1471-4159
DOI:10.1046/j.1471-4159.1998.70041411.x