Detection of an Interleukin-1 Intracellular Receptor Antagonist mRNA Variant

At least two versions of interleukin-1 receptor antagonist generated by alternative splicing are found intracellularly, but their functions remain poorly characterized. During studies aimed at characterizing the expression of these transcripts in human articular cartilage, we detected a variant cDNA...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1998-03, Vol.244 (1), p.91-95
Main Authors: Weissbach, Lawrence, Tran, Khoa, Colquhoun, Scott A., Champliaud, Marie-France, Towle, Christine A.
Format: Article
Language:English
Subjects:
Alternative Splicing
Amino Acid Sequence
Base Sequence
Cartilage, Articular - metabolism
Cells, Cultured
DNA, Complementary - isolation & purification
Exons
Humans
Interleukin 1 Receptor Antagonist Protein
Intracellular Fluid - metabolism
Keratinocytes - metabolism
Molecular Sequence Data
Osteoarthritis
Polymerase Chain Reaction
Receptors, Interleukin-1 - antagonists & inhibitors
Receptors, Interleukin-1 - genetics
RNA, Messenger - isolation & purification
RNA, Messenger - metabolism
Sialoglycoproteins - genetics
Sialoglycoproteins - isolation & purification
Sialoglycoproteins - metabolism
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Summary:At least two versions of interleukin-1 receptor antagonist generated by alternative splicing are found intracellularly, but their functions remain poorly characterized. During studies aimed at characterizing the expression of these transcripts in human articular cartilage, we detected a variant cDNA species that contained an additional 171 nucleotides within the type I interleukin-1 intracellular receptor antagonist cDNA which interrupted the coding region. This mRNA variant was also found to be expressed in keratinocytes. Translation likely initiates at an alternate methionine codon than that utilized for the previously reported interleukin-1 intracellular receptor antagonist isoforms, suggesting that this mRNA variant encodes a novel polypeptide that may play a role in interleukin-1 signaling.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.8217