Peptide Immunogen Mimicry of a Protein-Specific Structural Epitope on Human Choriogonadotropin

It is a challenge to construct synthetic immunogens that elicit antibodies (Abs) both directed to conformational epitopes and specific for a complex protein like human choriogonadotropin (hCG). A monoclonal antibody specific for hCG bound to regions around Lys$^{45}$ of the α subunit (hCGα) and Asp$...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1990-05, Vol.248 (4956), p.736-739
Main Authors: Bidart, Jean-Michel, Troalen, Federic, Ghillani, Pascale, Rouas, Nathalie, Razafindratsita, Alain, Bohuon, Claude, Bellet, Dominique
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Antibodies
Antigen-Antibody Complex
Antigenic determinants, haptens, artificial antigens
Antigens
Antiserum
Biological and medical sciences
Chorionic Gonadotropin - genetics
Chorionic Gonadotropin - immunology
Chorionic Gonadotropin, beta Subunit, Human
Epitopes
Epitopes - analysis
Epitopes - genetics
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Glycoprotein Hormones, alpha Subunit - immunology
Hormones
Humans
Immune Sera
Immunization
Laboratory rabbits
Lysine
Medical research
Molecular immunology
Molecular Sequence Data
Peptide Fragments - immunology
Peptide hormones
Protein Conformation
Proteins
Rabbits - immunology
Rabbits as laboratory animals
Receptors
Sequence Homology, Nucleic Acid
Sheep
Synthetic vaccines
Vaccination
Vaccines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c700t-356a9e248b3c08d7c96fd3d9cdab55ea2dbc48f79f7429d9bb0e65c8a4b09cd13
cites cdi_FETCH-LOGICAL-c700t-356a9e248b3c08d7c96fd3d9cdab55ea2dbc48f79f7429d9bb0e65c8a4b09cd13
container_end_page 739
container_issue 4956
container_start_page 736
container_title Science (American Association for the Advancement of Science)
container_volume 248
creator Bidart, Jean-Michel
Troalen, Federic
Ghillani, Pascale
Rouas, Nathalie
Razafindratsita, Alain
Bohuon, Claude
Bellet, Dominique
description It is a challenge to construct synthetic immunogens that elicit antibodies (Abs) both directed to conformational epitopes and specific for a complex protein like human choriogonadotropin (hCG). A monoclonal antibody specific for hCG bound to regions around Lys$^{45}$ of the α subunit (hCGα) and Asp$^{112}$ of the β subunit (hCGβ). A peptide comprising residues 46 to 55 of hCGα and residues 106 to 116 of hCGβ elicited Abs in rabbits that were directed to a discontinuous epitope and were specific for hCG. These Abs inhibited the binding of hCG to its receptor. Thus, a synthetic immunogen can mimic a conformational-specific epitope and can be useful for vaccine development.
doi_str_mv 10.1126/science.1692160
format article
fullrecord <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_79752558</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A9041119</galeid><jstor_id>2874142</jstor_id><sourcerecordid>A9041119</sourcerecordid><originalsourceid>FETCH-LOGICAL-c700t-356a9e248b3c08d7c96fd3d9cdab55ea2dbc48f79f7429d9bb0e65c8a4b09cd13</originalsourceid><addsrcrecordid>eNqN0s9v0zAUB_AIgUYZnLmAFCHBDiObf8ROfNyq0VUqdFKBI5HjvBRXiR3sRNr-e1w12gaqoPLBkr8fP8nPL4peY3SGMeHnXmkwCs4wFwRz9CSaYCRYIgiiT6MJQpQnOcrY8-iF9xuEQiboUXQ08kn04wa6XlcQz9t2MHYNJv6sW63cXWzrWMY3zvagTbLqQOlaq3jVu0H1g5NNfNXp3nYQWxNfD6008fSnddqurZGV7Z3ttHkZPatl4-HVuB9H3z5dfZ1eJ4vlbD69WCQqQ6hPKONSAEnzkiqUV5kSvK5oJVQlS8ZAkqpUaV5nos5SIipRlgg4U7lMSxQQpsfRh13dztlfA_i-aLVX0DTSgB18kYmMEcbyAE_-DVNKBc5T9N-SmHFOeL4t-e4vuLGDM-G5BcGUpYyTbbXTHVrLBgpt6tAgqUK7IXTSGqh1OL4QKMUYi6A_7tFhVRD-Zg8_-YMH0cNtv5aD98V89eVQufx-qLycHSjz2eKxPN0nlW0aWEMRJmK6fKzPd1o5672DuuicbqW7KzAqtsNfjMNfjNMcbrwdf2IoW6ge_H3-fsylV7KpnTRK-wcmaMoyngX3Zuc2vrfuPid5luKU0N_ZDhNs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213545620</pqid></control><display><type>article</type><title>Peptide Immunogen Mimicry of a Protein-Specific Structural Epitope on Human Choriogonadotropin</title><source>American Association for the Advancement of Science</source><source>JSTOR Archival Journals and Primary Sources Collection</source><source>Social Science Premium Collection</source><source>Education Collection</source><creator>Bidart, Jean-Michel ; Troalen, Federic ; Ghillani, Pascale ; Rouas, Nathalie ; Razafindratsita, Alain ; Bohuon, Claude ; Bellet, Dominique</creator><creatorcontrib>Bidart, Jean-Michel ; Troalen, Federic ; Ghillani, Pascale ; Rouas, Nathalie ; Razafindratsita, Alain ; Bohuon, Claude ; Bellet, Dominique</creatorcontrib><description>It is a challenge to construct synthetic immunogens that elicit antibodies (Abs) both directed to conformational epitopes and specific for a complex protein like human choriogonadotropin (hCG). A monoclonal antibody specific for hCG bound to regions around Lys$^{45}$ of the α subunit (hCGα) and Asp$^{112}$ of the β subunit (hCGβ). A peptide comprising residues 46 to 55 of hCGα and residues 106 to 116 of hCGβ elicited Abs in rabbits that were directed to a discontinuous epitope and were specific for hCG. These Abs inhibited the binding of hCG to its receptor. Thus, a synthetic immunogen can mimic a conformational-specific epitope and can be useful for vaccine development.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1692160</identifier><identifier>PMID: 1692160</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Amino Acid Sequence ; Amino acids ; Animals ; Antibodies ; Antigen-Antibody Complex ; Antigenic determinants, haptens, artificial antigens ; Antigens ; Antiserum ; Biological and medical sciences ; Chorionic Gonadotropin - genetics ; Chorionic Gonadotropin - immunology ; Chorionic Gonadotropin, beta Subunit, Human ; Epitopes ; Epitopes - analysis ; Epitopes - genetics ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Glycoprotein Hormones, alpha Subunit - immunology ; Hormones ; Humans ; Immune Sera ; Immunization ; Laboratory rabbits ; Lysine ; Medical research ; Molecular immunology ; Molecular Sequence Data ; Peptide Fragments - immunology ; Peptide hormones ; Protein Conformation ; Proteins ; Rabbits - immunology ; Rabbits as laboratory animals ; Receptors ; Sequence Homology, Nucleic Acid ; Sheep ; Synthetic vaccines ; Vaccination ; Vaccines</subject><ispartof>Science (American Association for the Advancement of Science), 1990-05, Vol.248 (4956), p.736-739</ispartof><rights>Copyright 1990 American Association for the Advancement of Science</rights><rights>1991 INIST-CNRS</rights><rights>COPYRIGHT 1990 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1990 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science May 11, 1990</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c700t-356a9e248b3c08d7c96fd3d9cdab55ea2dbc48f79f7429d9bb0e65c8a4b09cd13</citedby><cites>FETCH-LOGICAL-c700t-356a9e248b3c08d7c96fd3d9cdab55ea2dbc48f79f7429d9bb0e65c8a4b09cd13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/213545620/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/213545620?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,2884,2885,21378,21394,27924,27925,33611,33612,33877,33878,43733,43880,58238,58471,74221,74397</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=19345767$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1692160$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bidart, Jean-Michel</creatorcontrib><creatorcontrib>Troalen, Federic</creatorcontrib><creatorcontrib>Ghillani, Pascale</creatorcontrib><creatorcontrib>Rouas, Nathalie</creatorcontrib><creatorcontrib>Razafindratsita, Alain</creatorcontrib><creatorcontrib>Bohuon, Claude</creatorcontrib><creatorcontrib>Bellet, Dominique</creatorcontrib><title>Peptide Immunogen Mimicry of a Protein-Specific Structural Epitope on Human Choriogonadotropin</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>It is a challenge to construct synthetic immunogens that elicit antibodies (Abs) both directed to conformational epitopes and specific for a complex protein like human choriogonadotropin (hCG). A monoclonal antibody specific for hCG bound to regions around Lys$^{45}$ of the α subunit (hCGα) and Asp$^{112}$ of the β subunit (hCGβ). A peptide comprising residues 46 to 55 of hCGα and residues 106 to 116 of hCGβ elicited Abs in rabbits that were directed to a discontinuous epitope and were specific for hCG. These Abs inhibited the binding of hCG to its receptor. Thus, a synthetic immunogen can mimic a conformational-specific epitope and can be useful for vaccine development.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigen-Antibody Complex</subject><subject>Antigenic determinants, haptens, artificial antigens</subject><subject>Antigens</subject><subject>Antiserum</subject><subject>Biological and medical sciences</subject><subject>Chorionic Gonadotropin - genetics</subject><subject>Chorionic Gonadotropin - immunology</subject><subject>Chorionic Gonadotropin, beta Subunit, Human</subject><subject>Epitopes</subject><subject>Epitopes - analysis</subject><subject>Epitopes - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glycoprotein Hormones, alpha Subunit - immunology</subject><subject>Hormones</subject><subject>Humans</subject><subject>Immune Sera</subject><subject>Immunization</subject><subject>Laboratory rabbits</subject><subject>Lysine</subject><subject>Medical research</subject><subject>Molecular immunology</subject><subject>Molecular Sequence Data</subject><subject>Peptide Fragments - immunology</subject><subject>Peptide hormones</subject><subject>Protein Conformation</subject><subject>Proteins</subject><subject>Rabbits - immunology</subject><subject>Rabbits as laboratory animals</subject><subject>Receptors</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Sheep</subject><subject>Synthetic vaccines</subject><subject>Vaccination</subject><subject>Vaccines</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>ALSLI</sourceid><sourceid>CJNVE</sourceid><sourceid>M0P</sourceid><recordid>eNqN0s9v0zAUB_AIgUYZnLmAFCHBDiObf8ROfNyq0VUqdFKBI5HjvBRXiR3sRNr-e1w12gaqoPLBkr8fP8nPL4peY3SGMeHnXmkwCs4wFwRz9CSaYCRYIgiiT6MJQpQnOcrY8-iF9xuEQiboUXQ08kn04wa6XlcQz9t2MHYNJv6sW63cXWzrWMY3zvagTbLqQOlaq3jVu0H1g5NNfNXp3nYQWxNfD6008fSnddqurZGV7Z3ttHkZPatl4-HVuB9H3z5dfZ1eJ4vlbD69WCQqQ6hPKONSAEnzkiqUV5kSvK5oJVQlS8ZAkqpUaV5nos5SIipRlgg4U7lMSxQQpsfRh13dztlfA_i-aLVX0DTSgB18kYmMEcbyAE_-DVNKBc5T9N-SmHFOeL4t-e4vuLGDM-G5BcGUpYyTbbXTHVrLBgpt6tAgqUK7IXTSGqh1OL4QKMUYi6A_7tFhVRD-Zg8_-YMH0cNtv5aD98V89eVQufx-qLycHSjz2eKxPN0nlW0aWEMRJmK6fKzPd1o5672DuuicbqW7KzAqtsNfjMNfjNMcbrwdf2IoW6ge_H3-fsylV7KpnTRK-wcmaMoyngX3Zuc2vrfuPid5luKU0N_ZDhNs</recordid><startdate>19900511</startdate><enddate>19900511</enddate><creator>Bidart, Jean-Michel</creator><creator>Troalen, Federic</creator><creator>Ghillani, Pascale</creator><creator>Rouas, Nathalie</creator><creator>Razafindratsita, Alain</creator><creator>Bohuon, Claude</creator><creator>Bellet, Dominique</creator><general>American Society for the Advancement of Science</general><general>American Association for the Advancement of Science</general><general>The American Association for the Advancement of Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>IBG</scope><scope>IOV</scope><scope>ISN</scope><scope>0-V</scope><scope>3V.</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88B</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CJNVE</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9-</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0K</scope><scope>M0P</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEDU</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>7T5</scope><scope>7X8</scope></search><sort><creationdate>19900511</creationdate><title>Peptide Immunogen Mimicry of a Protein-Specific Structural Epitope on Human Choriogonadotropin</title><author>Bidart, Jean-Michel ; Troalen, Federic ; Ghillani, Pascale ; Rouas, Nathalie ; Razafindratsita, Alain ; Bohuon, Claude ; Bellet, Dominique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c700t-356a9e248b3c08d7c96fd3d9cdab55ea2dbc48f79f7429d9bb0e65c8a4b09cd13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigen-Antibody Complex</topic><topic>Antigenic determinants, haptens, artificial antigens</topic><topic>Antigens</topic><topic>Antiserum</topic><topic>Biological and medical sciences</topic><topic>Chorionic Gonadotropin - genetics</topic><topic>Chorionic Gonadotropin - immunology</topic><topic>Chorionic Gonadotropin, beta Subunit, Human</topic><topic>Epitopes</topic><topic>Epitopes - analysis</topic><topic>Epitopes - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Glycoprotein Hormones, alpha Subunit - immunology</topic><topic>Hormones</topic><topic>Humans</topic><topic>Immune Sera</topic><topic>Immunization</topic><topic>Laboratory rabbits</topic><topic>Lysine</topic><topic>Medical research</topic><topic>Molecular immunology</topic><topic>Molecular Sequence Data</topic><topic>Peptide Fragments - immunology</topic><topic>Peptide hormones</topic><topic>Protein Conformation</topic><topic>Proteins</topic><topic>Rabbits - immunology</topic><topic>Rabbits as laboratory animals</topic><topic>Receptors</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Sheep</topic><topic>Synthetic vaccines</topic><topic>Vaccination</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bidart, Jean-Michel</creatorcontrib><creatorcontrib>Troalen, Federic</creatorcontrib><creatorcontrib>Ghillani, Pascale</creatorcontrib><creatorcontrib>Rouas, Nathalie</creatorcontrib><creatorcontrib>Razafindratsita, Alain</creatorcontrib><creatorcontrib>Bohuon, Claude</creatorcontrib><creatorcontrib>Bellet, Dominique</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: High School</collection><collection>Gale in Context : Biography</collection><collection>Opposing Viewpoints Resource Center</collection><collection>Gale In Context: Canada</collection><collection>ProQuest Social Sciences Premium Collection【Remote access available】</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics &amp; Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest_Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Education Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Social Science Premium Collection</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>eLibrary</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric &amp; Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Education Collection</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology &amp; Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts – Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Agriculture Science Database</collection><collection>Education Database (ProQuest)</collection><collection>ProQuest Consumer Health Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric &amp; Aquatic Science Database</collection><collection>Materials science collection</collection><collection>ProQuest One Education</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>University of Michigan</collection><collection>Genetics Abstracts</collection><collection>Immunology Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bidart, Jean-Michel</au><au>Troalen, Federic</au><au>Ghillani, Pascale</au><au>Rouas, Nathalie</au><au>Razafindratsita, Alain</au><au>Bohuon, Claude</au><au>Bellet, Dominique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peptide Immunogen Mimicry of a Protein-Specific Structural Epitope on Human Choriogonadotropin</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1990-05-11</date><risdate>1990</risdate><volume>248</volume><issue>4956</issue><spage>736</spage><epage>739</epage><pages>736-739</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>It is a challenge to construct synthetic immunogens that elicit antibodies (Abs) both directed to conformational epitopes and specific for a complex protein like human choriogonadotropin (hCG). A monoclonal antibody specific for hCG bound to regions around Lys$^{45}$ of the α subunit (hCGα) and Asp$^{112}$ of the β subunit (hCGβ). A peptide comprising residues 46 to 55 of hCGα and residues 106 to 116 of hCGβ elicited Abs in rabbits that were directed to a discontinuous epitope and were specific for hCG. These Abs inhibited the binding of hCG to its receptor. Thus, a synthetic immunogen can mimic a conformational-specific epitope and can be useful for vaccine development.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>1692160</pmid><doi>10.1126/science.1692160</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0036-8075
ispartof Science (American Association for the Advancement of Science), 1990-05, Vol.248 (4956), p.736-739
issn 0036-8075
1095-9203
language eng
recordid cdi_proquest_miscellaneous_79752558
source American Association for the Advancement of Science; JSTOR Archival Journals and Primary Sources Collection; Social Science Premium Collection; Education Collection
subjects Amino Acid Sequence
Amino acids
Animals
Antibodies
Antigen-Antibody Complex
Antigenic determinants, haptens, artificial antigens
Antigens
Antiserum
Biological and medical sciences
Chorionic Gonadotropin - genetics
Chorionic Gonadotropin - immunology
Chorionic Gonadotropin, beta Subunit, Human
Epitopes
Epitopes - analysis
Epitopes - genetics
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Glycoprotein Hormones, alpha Subunit - immunology
Hormones
Humans
Immune Sera
Immunization
Laboratory rabbits
Lysine
Medical research
Molecular immunology
Molecular Sequence Data
Peptide Fragments - immunology
Peptide hormones
Protein Conformation
Proteins
Rabbits - immunology
Rabbits as laboratory animals
Receptors
Sequence Homology, Nucleic Acid
Sheep
Synthetic vaccines
Vaccination
Vaccines
title Peptide Immunogen Mimicry of a Protein-Specific Structural Epitope on Human Choriogonadotropin
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T13%3A40%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Peptide%20Immunogen%20Mimicry%20of%20a%20Protein-Specific%20Structural%20Epitope%20on%20Human%20Choriogonadotropin&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=Bidart,%20Jean-Michel&rft.date=1990-05-11&rft.volume=248&rft.issue=4956&rft.spage=736&rft.epage=739&rft.pages=736-739&rft.issn=0036-8075&rft.eissn=1095-9203&rft.coden=SCIEAS&rft_id=info:doi/10.1126/science.1692160&rft_dat=%3Cgale_proqu%3EA9041119%3C/gale_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c700t-356a9e248b3c08d7c96fd3d9cdab55ea2dbc48f79f7429d9bb0e65c8a4b09cd13%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=213545620&rft_id=info:pmid/1692160&rft_galeid=A9041119&rft_jstor_id=2874142&rfr_iscdi=true