High levels of gelatinase-B and active gelatinase-A in metastatic glioblastoma

• Published in: Journal of neuro-oncology 1998, Vol.36 (1), p.21-29
• Main Authors: FORSYTH, P. A, LAING, T. D, GIBSON, A. W, REWCASTLE, N. B, BRASHER, P, SUTHERLAND, G, JOHNSTON, R. N, EDWARDS, D. R
• Format: Article
• Language: English
• Subjects: Adult; Astrocytes; Biological and medical sciences; Brain cancer; Brain Neoplasms - enzymology; Brain tumors; Collagenases - metabolism; Enzyme Activation; Fatal Outcome; Gelatin; Gelatinase; Gelatinases - metabolism; Glioblastoma; Glioblastoma - enzymology; Glioblastoma - secondary; Glioma; Head and Neck Neoplasms - enzymology; Head and Neck Neoplasms - secondary; Humans; Lymphatic Metastasis; Male; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Medical sciences; Metalloendopeptidases - metabolism; Metastases; Metastasis; Neurology; Orbital Neoplasms - enzymology; Orbital Neoplasms - secondary; Skull Neoplasms - enzymology; Skull Neoplasms - secondary; Tracheal Neoplasms - enzymology; Tracheal Neoplasms - secondary; Tumors of the nervous system. Phacomatoses
• ISSN: 0167-594X; 1573-7373
• DOI: 10.1023/A:1005879027267

Extra-neural metastases from glioblastoma multiforme (GBM) are rare. Because gelatinases-A and -B have been implicated in tumor invasion/metastasis in non-neural tumors, we compared the expression of gelatinase-A and -B in 2 patients (both had a prior craniotomy performed) with extraneural metastases from GBM to expression levels in 24 other gliomas; 15 non-metastatic GBMs, 9 other lower grade gliomas, and 7 normal brain tissues. The intracerebral tumor from both patients, patient # 1's extraneural metastases, 24 other gliomas, 1 sample of reactive astrocytes and 7 normal brain tissues were studied using gelatin zymography. The active form of gelatinases was confirmed by co-migration after activation with APMA. Expression of the latent form of gelatinase-A correlated with glioma grade (r = 0.486; p = 0.0053). Active gelatinase-A was found only in the 2 GBMs with extraneural metastases and patient # 1's cervical metastases. In contrast, latent gelatinase-B levels correlated more strongly with histologic grade (r = 0.577; p = 0.0009) (higher levels with higher grades). Very high levels of gelatinase-B were seen in both GBMs with extraneural metastases, a cervical extraneural metastases, and 2 GBMs without metastases. We observed that gelatinases-A and -B are present in most gliomas but we found active gelatinase-A only in the GBMs with extraneural metastases suggesting that the active form of this enzyme may determine the metastatic potential of GBMs. We propose that high levels of gelatinolytic activities are associated with intracerebral invasion and rarely, metastases of GBMs.