Estrogen regulation of placental alkaline phosphatase gene expression in a human endometrial adenocarcinoma cell line

A human endometrial tumor (Ishikawa) cell line in culture responded to estradiol stimulation, as measured by growth and alkaline phosphatase activity. These effects were similar whether the medium was enriched with serum or was serum-free. Estradiol increased placental alkaline phosphatase activity...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1990-06, Vol.50 (11), p.3306-3310
Main Authors: ALBERT, J. L, SUNDSTROM, S. A, LYTTLE, C. R
Format: Article
Language:English
Subjects:
Adenocarcinoma - enzymology
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Alkaline Phosphatase - genetics
Alkaline Phosphatase - metabolism
Biological and medical sciences
Cell Count
Estradiol - analogs & derivatives
Estradiol - pharmacology
Estrogen Antagonists - pharmacology
Female
Female genital diseases
Gene Expression Regulation, Enzymologic - drug effects
Gene Expression Regulation, Neoplastic - drug effects
Gynecology. Andrology. Obstetrics
Humans
Isoenzymes - genetics
Isoenzymes - metabolism
Medical sciences
Placenta - enzymology
Polyunsaturated Alkamides
RNA, Messenger - analysis
Tumor Cells, Cultured - drug effects
Tumor Cells, Cultured - pathology
Tumors
Uterine Neoplasms - enzymology
Uterine Neoplasms - genetics
Uterine Neoplasms - pathology
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Summary:A human endometrial tumor (Ishikawa) cell line in culture responded to estradiol stimulation, as measured by growth and alkaline phosphatase activity. These effects were similar whether the medium was enriched with serum or was serum-free. Estradiol increased placental alkaline phosphatase activity 2-3-fold over control in these Ishikawa cells. The mechanism for this increase appeared to be at the level of transcription, at least in part, since there was an increase in the concentration of placental alkaline phosphatase mRNA. The administration of tamoxifen or 4-hydroxytamoxifen was unable to antagonize the estradiol-stimulated alkaline phosphatase enzyme activity or mRNA expression. The administration of tamoxifen alone had no effect on alkaline phosphatase enzyme activity, but tamoxifen did stimulate the steady state concentration of alkaline phosphatase mRNA. In contrast, a new antiestrogen, ICI 164,384, was able to antagonize both of these estradiol-stimulated effects.
ISSN:0008-5472
1538-7445