Sarcocystis gigantea lectin ― mitogen and polyclonal B-cell activator

The present study further examined the in vitro response of human mononuclear cells (MNC) to the Sarcocystis gigantea lectin (SGL). The results confirm our previous report that SGL is mitogenic for human MNC. We now report that SGL is not only a potent mitogen but also a polyclonal activator for hum...

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Published in:Parasitology Research 1990-01, Vol.76 (4), p.332-335
Main Authors: TIETZ, H. J, MONTAG, T, BROSE, E, WIDERA, P, KIESSIG, S. T, MANN, W, HIEPE, T
Format: Article
Language:English
Subjects:
Animals
B-Lymphocytes - immunology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Immunoenzyme Techniques
Immunoglobulin G - biosynthesis
Immunoglobulin M - biosynthesis
Kinetics
Lectins - immunology
Leukocytes, Mononuclear - immunology
Lymphocyte Activation
Mitogens - immunology
Modulation of the immune response (stimulation, suppression)
Pokeweed Mitogens - immunology
Sarcocystis - immunology
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Summary:The present study further examined the in vitro response of human mononuclear cells (MNC) to the Sarcocystis gigantea lectin (SGL). The results confirm our previous report that SGL is mitogenic for human MNC. We now report that SGL is not only a potent mitogen but also a polyclonal activator for human peripheral B cells. As was true for pokeweed mitogen (PWM, 2 micrograms/ml), the addition of SGL (25 micrograms protein/ml) to cultures of MNC caused lymphocyte proliferation and B-cell maturation, indicated by a marked increase in IgG and IgM production. As measured by the [3H]-thymidine incorporation assay, SGL induced significantly higher proliferative responses than PWM (P less than 0.01, n = 24). The values obtained by SGL and PWM for IgG and IgM synthesis were essentially identical. As opposed to SGL, the sarcotoxin-containing fraction (SGTF) did not induce antibody formation or proliferative responses in human MNC.
ISSN:0932-0113
0044-3255
1432-1955
DOI:10.1007/BF00928188