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Age-related differences in response to neutrophil-mediated reperfusion injury in the neonatal piglet heart
Background: Neonatal hearts have altered adhesion molecule interactions in response to ischemia-reperfusion. How this affects myocardial function is unknown. Methods: Isolated, buffer perfused 0- to 2-day (newborn) and 2-week piglet hearts were first subjected to 20-minute global, normothermic ische...
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Published in: | Surgery 1998-03, Vol.123 (3), p.294-304 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Background: Neonatal hearts have altered adhesion molecule interactions in response to ischemia-reperfusion. How this affects myocardial function is unknown.
Methods: Isolated, buffer perfused 0- to 2-day (newborn) and 2-week piglet hearts were first subjected to 20-minute global, normothermic ischemia, followed by 45 minutes of reperfusion during which 150 × 10
6 newborn or 2-week neutrophils were infused. In some hearts, an antibody to SLe
x (CSLEX-1) was infused with neutrophils during reperfusion. Hemodynamic variables, including left ventricular developed pressure (LVDP), were recorded at timed intervals. Neutrophil CD-18, L-selectin, and SLe
x contents were measured by flow cytometry.
Results: Full recovery of LVDP was observed in newborn hearts receiving newborn or 2-week-old neutrophils. Recovery of LVDP was depressed (
p < 0.01, ANOVA) in 2-week-old hearts receiving 2-week old, not newborn, neutrophils. Infusion of CSLEX-1 in 2-week-old hearts restored LVDP to baseline. Whereas flow cytometry showed higher (
p < 0.01, Student's
t test) CD-18 and L-selectin expression on newborn versus 2-week-old neutrophils, newborn neutrophils expressed lower (
p < 0.01) SLe
x levels.
Conclusions: Initial “loose” neutrophil-endothelial selectin interactions are a necessary prelude to “firm” adhesion and reperfusion injury. Operations performed soon after birth may be better tolerated than when surgery is delayed; anti-SLe
x preparations may prove beneficial when performing cardiac procedures on older infants. (Surgery 1998;123:294-304) |
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ISSN: | 0039-6060 1532-7361 |
DOI: | 10.1016/S0039-6060(98)70182-5 |