Sustained decrease of peripheral lymphocytes after allogeneic blood stem cell aphereses

• Published in: British journal of haematology 1998-03, Vol.100 (4), p.695-697
• Main Authors: Novotny, Jürgen, Kadar, Janos, Hertenstein, Bernd, Schröder, Detlev, Petersen, Detlev, Arseniev, Lubomir, Stangel, Walter, Ganser, Arnold
• Format: Article
• Language: English
• Subjects: allogeneic stem cell transplantation; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; Bone marrow, stem cells transplantation. Graft versus host reaction; donor safety; Female; haemopoietic progenitor cells; Hematology; Hematopoietic Stem Cell Transplantation - adverse effects; Humans; Leukapheresis - adverse effects; Leukocyte Count; long‐term effects; lymphocytes; Lymphopenia - etiology; Male; Medical sciences; Neutrophils; Platelet Count; Retrospective Studies; Transfusions. Complications. Transfusion reactions. Cell and gene therapy
• ISSN: 0007-1048; 1365-2141
• DOI: 10.1046/j.1365-2141.1998.00629.x

48 healthy donors underwent peripheral blood stem cell (PBSC) apheresis for allogeneic transplantation beginning on day 4 of G‐CSF (2 × 5 μg/kg) mobilization. In one to four (median two) large‐volume mononuclear cell aphereses, a median of 55.9 × 109 of lymphocytes (range 21.0–109.2 × 109) were collected, an amount comparable to lymphocyte numbers removed by therapeutic lymphaphereses in autoimmune diseases. Mean peripheral lymphocyte counts decreased from premobilization values of 2.31 × 109/l to 1.31 × 109/l at a median of 34 d (1 month) and 1.53 × 109/l at a median of 327 d (11 months). The decrease in peripheral lymphocyte counts was significantly correlated with the number of lymphocytes removed and the number of aphereses. Neutrophil and platelet counts returned to normal values after 1 month whereas monocyte counts and haemoglobin concentrations were significantly decreased at 1 month but not at 11 months.