Rapid and transient expression of Ets2 in mature macrophages following stimulation with cMGF, LPS, and PKC activators

• Published in: Genes & development 1990-03, Vol.4 (3), p.401-409
• Main Authors: BOULUKOS, K. E, POGNONEC, P, SARIBAN, E, BAILLY, M, LAGROU, C, GHYSDAEL, J
• Format: Article
• Language: English
• Subjects: Animals; Avian Proteins; Biological and medical sciences; Cell Line, Transformed; Cells, Cultured; Chickens; DNA-Binding Proteins - metabolism; Enzyme Activation; Fundamental and applied biological sciences. Psychology; Gene Expression; Growth Substances - pharmacology; Humans; Intercellular Signaling Peptides and Proteins; Kinetics; Lipopolysaccharides - pharmacology; Macrophages - metabolism; Mice; Molecular and cellular biology; Molecular genetics; Monocytes - metabolism; Protein Kinase C - metabolism; Proto-Oncogene Proteins - biosynthesis; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; Proto-Oncogene Proteins c-ets; Proto-Oncogenes; RNA, Messenger - biosynthesis; Signal Transduction; Transcription Factors
• ISSN: 0890-9369; 1549-5477
• DOI: 10.1101/gad.4.3.401

We reported previously that Ets2 is expressed in normal and transformed macrophages. We show here that the expression of both c-ets-2 mRNA proteins is induced rapidly and transiently in chicken nondividing bone marrow-derived macrophages but not in E26-transformed myeloblasts in response to chicken myelomonocytic growth factor (cMGF), an avian hematopoietic growth factor required for survival, proliferation, and colony formation of avian myeloid cells. c-ets-2 expression is also rapidly induced in chicken bone marrow-derived macrophages, human monocytes, and mouse peritoneal macrophages in response to LPS and/or PKC activators. The rapid induction of Ets2 after treatment of chicken bone marrow-derived macrophages by cMGF is blunted after down-regulation or inactivation of PKC, suggesting a role of PKC in the cMGF-induced signal transduction pathway. Because Ets2 is localized in the nucleus of macrophages and binds to DNA in vitro, the kinetics of its expression suggest a role for Ets2 in the transduction within the nucleus of specific signals received at the cell membrane and involved in securing the survival and/or the development of functional competence of these cells.