A 36-kDa mitochondrial protein is responsible for cyanide-resistant respiration in Hansenula anomala

Antimycin A-dependent induction of cyanide-resistant respiration in Hansenula anomala was reversibly blocked by carbonylcyanide- m-chlorophenylhydrazone (CCCP). When the cells were pulse-labeled with [ 35S]methionine in the presence of both antimycin A and CCCP, the radioactivity was incorporated in...

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Bibliographic Details
Published in:FEBS letters 1990-05, Vol.264 (1), p.149-152
Main Authors: Minagawa, Nobuko, Sakajo, Shigeru, Komiyama, Tadazumi, Yoshimoto, Akio
Format: Article
Language:English
Subjects:
Alternative oxidase
Antimycin A - pharmacology
Biological and medical sciences
BSA, bovine serum albumin
Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology
CCCP, carbonylcyanide-m-chlorophenylhydrazone
Cell structures and functions
cyanide
Cyanide-resistant respiration
Fundamental and applied biological sciences. Psychology
Fungal Proteins - biosynthesis
Fungal Proteins - isolation & purification
Fungal Proteins - metabolism
Kinetics
Methionine - metabolism
Mitochondria
Mitochondria - drug effects
Mitochondria - metabolism
Mitochondria and cell respiration
Molecular and cellular biology
Molecular Weight
Nitriles - pharmacology
oxidase
Oxygen Consumption - drug effects
Pichia - metabolism
PMSF, phenylmethane sulfonylfluoride
Precursor
Protein import
respiration
Saccharomycetales - metabolism
Spheroplasts - drug effects
Spheroplasts - metabolism
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Summary:Antimycin A-dependent induction of cyanide-resistant respiration in Hansenula anomala was reversibly blocked by carbonylcyanide- m-chlorophenylhydrazone (CCCP). When the cells were pulse-labeled with [ 35S]methionine in the presence of both antimycin A and CCCP, the radioactivity was incorporated into a 39 kDa mitochondrial protein. Upon removal of CCCP, this protein was processed into a 36 kDa form. The increase in the 36 kDa protein completely paralleled that in cyanide-resistant respiration activity, suggesting that the 39 kDa protein is the precursor of the 36 kDa protein, which is responsible for cyanide-resistant respiration.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)80787-J