Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains

Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked beta-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively. The low-molecular-mass hydrolysis products of beta-glucan and xylan were compa...

Full description

Saved in:
Bibliographic Details
Published in:Applied microbiology and biotechnology 1998-02, Vol.49 (2), p.175-181
Main Authors: Jaskari, J, Kontula, P, Siitonen, A, Jousimies-Somer, H, Mattila-Sandholm, T, Poutanen, K
Format: Article
Language:English
Subjects:
Bacteriology
beta-Glucans
Bifidobacterium - growth & development
Bifidobacterium - metabolism
Biological and medical sciences
Biotechnology
Carbohydrate Metabolism
Chromatography, High Pressure Liquid
Fundamental and applied biological sciences. Psychology
Glucans - metabolism
Growth, nutrition, cell differenciation
intestinal microorganisms
Intestinal Mucosa - microbiology
Lactobacillus - growth & development
Lactobacillus - metabolism
Microbiology
Oligosaccharides - metabolism
Oligosaccharides - pharmacology
probiotic bacteria
Probiotics
Raffinose - metabolism
Raffinose - pharmacology
Substrate Specificity
Xylans - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked beta-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively. The low-molecular-mass hydrolysis products of beta-glucan and xylan were compared with fructooligomers and raffinose in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides, Clostridium and Escherichia coli) strains in vitro. A degradation profile of each carbohydrate and total sugar consumption were analysed with HPLC, and bacterial growth rate with an automatic turbidometer, the Bioscreen C system beta-Glucooligomers and xylooligomers both enhanced the growth of health-promoting probiotic strains as compared with intestinal bacterial growth, but not to a significant level. Raffinose stimulated the probiotic strains significantly, whereas fructooligomers induced high average growth for intestinal bacteria also.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530051155