Interaction of short-range repressors with Drosophila CtBP in the embryo

• Published in: Science (American Association for the Advancement of Science) 1998-04, Vol.280 (5360), p.101-104
• Main Authors: Nibu, Y. (University of California, Berkeley, CA.), Zhang, H, Levine, M
• Format: Article
• Language: English
• Subjects: Alcohol Oxidoreductases; Amino Acid Sequence; Amino acids; Animals; Animals, Genetically Modified; Biological and medical sciences; Biology; Cell Nucleus - metabolism; Classical genetics, quantitative genetics, hybrids; Coding; Complementary DNA; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; DROSOPHILA; Drosophila - embryology; Drosophila - genetics; Drosophila - metabolism; Drosophila Proteins; Embryo, Nonmammalian - metabolism; Embryos; Evolution; FACTEUR DE TRANSCRIPTION; FACTORES DE TRANSCRIPCION; Female; Fundamental and applied biological sciences. Psychology; GENE; GENE DOSAGE; Gene Expression Regulation; GENES; Genes, Insect; Genes, Reporter; Genetic mutation; Genetic transcription; Genetics of eukaryotes. Biological and molecular evolution; Humans; Insect Proteins - genetics; Insect Proteins - metabolism; Insects; Invertebrata; Literary Devices; Male; Mammals; Mating behavior; Molecular Sequence Data; Mutation; Observations; Phosphoproteins - chemistry; Phosphoproteins - genetics; Phosphoproteins - metabolism; Physiological aspects; Recombinant Fusion Proteins - metabolism; Repression; Repressor Proteins - chemistry; Repressor Proteins - genetics; Repressor Proteins - metabolism; Snail Family Transcription Factors; Snails; Stripes; TRANSCRIPCION; TRANSCRIPTION; Transcription (Genetics); TRANSCRIPTION FACTORS; Transcription, Genetic; Yeasts
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.280.5360.101

Human CtBP attenuates transcriptional activation and tumorigenesis mediated by the adenovirus E1A protein. The E1A sequence motif that interacts with CtBP, Pro-X-Asp-Leu-Ser-X-Lys (P-DLS-K), is present in the repression domains of two unrelated short-range repressors in Drosophila, Knirps and Snail, and is essential for the interaction of these proteins with Drosophila CtBP (dCtBP). A P-element-induced mutation in dCtBP exhibits gene-dosage interactions with a null mutation in knirps, which is consistent with the occurrence of Knirps-dCtBP interactions in vivo. These observations suggest that CtBP and dCtBP are engaged in an evolutionarily conserved mechanism of transcriptional repression, which is used in both Drosophila and mammals