Cloning of the PYR4 gene encoding orotidine-5'-phosphate decarboxylase in Cephalosporium acremonium

We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMP...

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Bibliographic Details
Published in:Current genetics 1990-03, Vol.17 (3), p.223-227
Main Authors: Vian, A, Penalva, M.A
Format: Article
Language:English
Subjects:
Acremonium - enzymology
Acremonium - genetics
Acremonium strictum
Amino Acid Sequence
Biological and medical sciences
Biological Evolution
carboxy-lyases
Carboxy-Lyases - genetics
cloning
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
genes
Genes, Fungal
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Neurospora crassa - genetics
Nucleic Acid Hybridization
Orotidine-5'-Phosphate Decarboxylase - genetics
Restriction Mapping
Sequence Homology, Nucleic Acid
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Summary:We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecase), and most probably does not contain introns. Maxicell analysis in E. coli shows that it encodes a 46 kDa polypeptide. The C. acremonium OMPdecase contains a highly conserved pentadecapeptide characteristic for this category of enzyme. Extensive sequence comparison suggests an important role of this region in enzymatic activity.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00312613