Loading…

Cloning of the PYR4 gene encoding orotidine-5'-phosphate decarboxylase in Cephalosporium acremonium

We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMP...

Full description

Saved in:

Bibliographic Details
Published in:	Current genetics 1990-03, Vol.17 (3), p.223-227
Main Authors:	Vian, A, Penalva, M.A
Format:	Article
Language:	English
Subjects:	Acremonium - enzymology Acremonium - genetics Acremonium strictum Amino Acid Sequence Biological and medical sciences Biological Evolution carboxy-lyases Carboxy-Lyases - genetics cloning Cloning, Molecular Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology genes Genes, Fungal Genes. Genome Molecular and cellular biology Molecular genetics Molecular Sequence Data Neurospora crassa - genetics Nucleic Acid Hybridization Orotidine-5'-Phosphate Decarboxylase - genetics Restriction Mapping Sequence Homology, Nucleic Acid
Citations:	Items that this one cites Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by	cdi_FETCH-LOGICAL-c366t-b2d18b5f3e244bf4b905a08ee3e11475c1e73801e228c0724591feea8fd31b363
cites	cdi_FETCH-LOGICAL-c366t-b2d18b5f3e244bf4b905a08ee3e11475c1e73801e228c0724591feea8fd31b363
container_end_page	227
container_issue	3
container_start_page	223
container_title	Current genetics
container_volume	17
creator	Vian, A Penalva, M.A
description	We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecase), and most probably does not contain introns. Maxicell analysis in E. coli shows that it encodes a 46 kDa polypeptide. The C. acremonium OMPdecase contains a highly conserved pentadecapeptide characteristic for this category of enzyme. Extensive sequence comparison suggests an important role of this region in enzymatic activity.
doi_str_mv	10.1007/BF00312613
format	article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79775831</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79775831</sourcerecordid><originalsourceid>FETCH-LOGICAL-c366t-b2d18b5f3e244bf4b905a08ee3e11475c1e73801e228c0724591feea8fd31b363</originalsourceid><addsrcrecordid>eNqF0MGL1DAUBvAgyjq7evEu5iAKQvW9pGmSow67Kiwo6h48lTR9mam0zZi04P73RmdYj57eg-_Hd_gYe4LwGgH0m3dXABJFg_Ie22AtRQXWyPtsA6hFZcDIh-w85x8AKIzVZ-xMYA3C2g3z2zHOw7zjMfBlT_zz9y8139FMnGYf-79JistQPqrUy-qwj_mwdwvxnrxLXfx1O7pMfJj5lkowljimYZ2484mm0r1Oj9iD4MZMj0_3gt1cXX7bfqiuP73_uH17XXnZNEvViR5Np4IkUdddqDsLyoEhkoRYa-WRtDSAJITxoEWtLAYiZ0IvsZONvGAvjr2HFH-ulJd2GrKncXQzxTW32mqtjMT_QlTaaItQ4Ksj9CnmnCi0hzRMLt22CO2f6dt_0xf89NS6dhP1d_S0dcmfn3KXvRtDcrMf8h1rjAapRGHPjiy42LpdKuTmqwCUIBprrVDyN_mMk1M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15787910</pqid></control><display><type>article</type><title>Cloning of the PYR4 gene encoding orotidine-5'-phosphate decarboxylase in Cephalosporium acremonium</title><source>Springer Online Journal Archives</source><creator>Vian, A ; Penalva, M.A</creator><creatorcontrib>Vian, A ; Penalva, M.A</creatorcontrib><description>We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecase), and most probably does not contain introns. Maxicell analysis in E. coli shows that it encodes a 46 kDa polypeptide. The C. acremonium OMPdecase contains a highly conserved pentadecapeptide characteristic for this category of enzyme. Extensive sequence comparison suggests an important role of this region in enzymatic activity.</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/BF00312613</identifier><identifier>PMID: 2140299</identifier><identifier>CODEN: CUGED5</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Acremonium - enzymology ; Acremonium - genetics ; Acremonium strictum ; Amino Acid Sequence ; Biological and medical sciences ; Biological Evolution ; carboxy-lyases ; Carboxy-Lyases - genetics ; cloning ; Cloning, Molecular ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; genes ; Genes, Fungal ; Genes. Genome ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Neurospora crassa - genetics ; Nucleic Acid Hybridization ; Orotidine-5'-Phosphate Decarboxylase - genetics ; Restriction Mapping ; Sequence Homology, Nucleic Acid</subject><ispartof>Current genetics, 1990-03, Vol.17 (3), p.223-227</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-b2d18b5f3e244bf4b905a08ee3e11475c1e73801e228c0724591feea8fd31b363</citedby><cites>FETCH-LOGICAL-c366t-b2d18b5f3e244bf4b905a08ee3e11475c1e73801e228c0724591feea8fd31b363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6870352$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2140299$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vian, A</creatorcontrib><creatorcontrib>Penalva, M.A</creatorcontrib><title>Cloning of the PYR4 gene encoding orotidine-5'-phosphate decarboxylase in Cephalosporium acremonium</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecase), and most probably does not contain introns. Maxicell analysis in E. coli shows that it encodes a 46 kDa polypeptide. The C. acremonium OMPdecase contains a highly conserved pentadecapeptide characteristic for this category of enzyme. Extensive sequence comparison suggests an important role of this region in enzymatic activity.</description><subject>Acremonium - enzymology</subject><subject>Acremonium - genetics</subject><subject>Acremonium strictum</subject><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological Evolution</subject><subject>carboxy-lyases</subject><subject>Carboxy-Lyases - genetics</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genes, Fungal</subject><subject>Genes. Genome</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Neurospora crassa - genetics</subject><subject>Nucleic Acid Hybridization</subject><subject>Orotidine-5'-Phosphate Decarboxylase - genetics</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNqF0MGL1DAUBvAgyjq7evEu5iAKQvW9pGmSow67Kiwo6h48lTR9mam0zZi04P73RmdYj57eg-_Hd_gYe4LwGgH0m3dXABJFg_Ie22AtRQXWyPtsA6hFZcDIh-w85x8AKIzVZ-xMYA3C2g3z2zHOw7zjMfBlT_zz9y8139FMnGYf-79JistQPqrUy-qwj_mwdwvxnrxLXfx1O7pMfJj5lkowljimYZ2484mm0r1Oj9iD4MZMj0_3gt1cXX7bfqiuP73_uH17XXnZNEvViR5Np4IkUdddqDsLyoEhkoRYa-WRtDSAJITxoEWtLAYiZ0IvsZONvGAvjr2HFH-ulJd2GrKncXQzxTW32mqtjMT_QlTaaItQ4Ksj9CnmnCi0hzRMLt22CO2f6dt_0xf89NS6dhP1d_S0dcmfn3KXvRtDcrMf8h1rjAapRGHPjiy42LpdKuTmqwCUIBprrVDyN_mMk1M</recordid><startdate>19900301</startdate><enddate>19900301</enddate><creator>Vian, A</creator><creator>Penalva, M.A</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19900301</creationdate><title>Cloning of the PYR4 gene encoding orotidine-5'-phosphate decarboxylase in Cephalosporium acremonium</title><author>Vian, A ; Penalva, M.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-b2d18b5f3e244bf4b905a08ee3e11475c1e73801e228c0724591feea8fd31b363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Acremonium - enzymology</topic><topic>Acremonium - genetics</topic><topic>Acremonium strictum</topic><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>Biological Evolution</topic><topic>carboxy-lyases</topic><topic>Carboxy-Lyases - genetics</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genes, Fungal</topic><topic>Genes. Genome</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Neurospora crassa - genetics</topic><topic>Nucleic Acid Hybridization</topic><topic>Orotidine-5'-Phosphate Decarboxylase - genetics</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vian, A</creatorcontrib><creatorcontrib>Penalva, M.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vian, A</au><au>Penalva, M.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning of the PYR4 gene encoding orotidine-5'-phosphate decarboxylase in Cephalosporium acremonium</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1990-03-01</date><risdate>1990</risdate><volume>17</volume><issue>3</issue><spage>223</spage><epage>227</epage><pages>223-227</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><coden>CUGED5</coden><abstract>We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecase), and most probably does not contain introns. Maxicell analysis in E. coli shows that it encodes a 46 kDa polypeptide. The C. acremonium OMPdecase contains a highly conserved pentadecapeptide characteristic for this category of enzyme. Extensive sequence comparison suggests an important role of this region in enzymatic activity.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>2140299</pmid><doi>10.1007/BF00312613</doi><tpages>5</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0172-8083
ispartof	Current genetics, 1990-03, Vol.17 (3), p.223-227
issn	0172-8083 1432-0983
language	eng
recordid	cdi_proquest_miscellaneous_79775831
source	Springer Online Journal Archives
subjects	Acremonium - enzymology Acremonium - genetics Acremonium strictum Amino Acid Sequence Biological and medical sciences Biological Evolution carboxy-lyases Carboxy-Lyases - genetics cloning Cloning, Molecular Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology genes Genes, Fungal Genes. Genome Molecular and cellular biology Molecular genetics Molecular Sequence Data Neurospora crassa - genetics Nucleic Acid Hybridization Orotidine-5'-Phosphate Decarboxylase - genetics Restriction Mapping Sequence Homology, Nucleic Acid
title	Cloning of the PYR4 gene encoding orotidine-5'-phosphate decarboxylase in Cephalosporium acremonium
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T14%3A12%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20of%20the%20PYR4%20gene%20encoding%20orotidine-5'-phosphate%20decarboxylase%20in%20Cephalosporium%20acremonium&rft.jtitle=Current%20genetics&rft.au=Vian,%20A&rft.date=1990-03-01&rft.volume=17&rft.issue=3&rft.spage=223&rft.epage=227&rft.pages=223-227&rft.issn=0172-8083&rft.eissn=1432-0983&rft.coden=CUGED5&rft_id=info:doi/10.1007/BF00312613&rft_dat=%3Cproquest_cross%3E79775831%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c366t-b2d18b5f3e244bf4b905a08ee3e11475c1e73801e228c0724591feea8fd31b363%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=15787910&rft_id=info:pmid/2140299&rfr_iscdi=true