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Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein
We previously established a novel mouse model for human aging and identified the genetic foundation responsible for it. A defect in expression of a novel gene, termed klotho ( kl), leads to a syndrome resembling human aging in mice. The kl gene encodes a single-pass membrane protein whose extracellu...
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Published in: | FEBS letters 1998-03, Vol.424 (1), p.6-10 |
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container_title | FEBS letters |
container_volume | 424 |
creator | Shiraki-Iida, Takako Aizawa, Hiroki Matsumura, Yutaka Sekine, Susumu Iida, Akihiro Anazawa, Hideharu Nagai, Ryozo Kuro-o, Makoto Nabeshima, Yo-ichi |
description | We previously established a novel mouse model for human aging and identified the genetic foundation responsible for it. A defect in expression of a novel gene, termed
klotho (
kl), leads to a syndrome resembling human aging in mice. The
kl gene encodes a single-pass membrane protein whose extracellular domain carries homology to
β-glucosidases. In this report, we present the entire mouse
kl gene organization. The mouse
kl gene spans about 50 kilobases and consists of five exons. The promoter region lacks a TATA-box and contains four potential binding sites for SP1. We further show that two
kl gene transcripts encoding membrane or secreted protein are generated through alternative transcriptional termination. These findings provide fundamental information for further study of the
kl gene which may regulate aging in vivo. |
doi_str_mv | 10.1016/S0014-5793(98)00127-6 |
format | article |
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klotho (
kl), leads to a syndrome resembling human aging in mice. The
kl gene encodes a single-pass membrane protein whose extracellular domain carries homology to
β-glucosidases. In this report, we present the entire mouse
kl gene organization. The mouse
kl gene spans about 50 kilobases and consists of five exons. The promoter region lacks a TATA-box and contains four potential binding sites for SP1. We further show that two
kl gene transcripts encoding membrane or secreted protein are generated through alternative transcriptional termination. These findings provide fundamental information for further study of the
kl gene which may regulate aging in vivo.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(98)00127-6</identifier><identifier>PMID: 9537505</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Alternative Splicing ; Alternative transcriptional termination ; Amino Acid Sequence ; Animals ; Base Sequence ; Competitive polymerase chain reaction ; Glucuronidase ; Humans ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Mice ; Molecular Sequence Data ; Mouse klotho gene ; Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Transcription, Genetic</subject><ispartof>FEBS letters, 1998-03, Vol.424 (1), p.6-10</ispartof><rights>1998 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c314t-5271b7aabdd2e9105ee74003ec11ff23776c59e3593c81158cbac18b45d41aaf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579398001276$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3536,27901,27902,45756</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9537505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shiraki-Iida, Takako</creatorcontrib><creatorcontrib>Aizawa, Hiroki</creatorcontrib><creatorcontrib>Matsumura, Yutaka</creatorcontrib><creatorcontrib>Sekine, Susumu</creatorcontrib><creatorcontrib>Iida, Akihiro</creatorcontrib><creatorcontrib>Anazawa, Hideharu</creatorcontrib><creatorcontrib>Nagai, Ryozo</creatorcontrib><creatorcontrib>Kuro-o, Makoto</creatorcontrib><creatorcontrib>Nabeshima, Yo-ichi</creatorcontrib><title>Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We previously established a novel mouse model for human aging and identified the genetic foundation responsible for it. A defect in expression of a novel gene, termed
klotho (
kl), leads to a syndrome resembling human aging in mice. The
kl gene encodes a single-pass membrane protein whose extracellular domain carries homology to
β-glucosidases. In this report, we present the entire mouse
kl gene organization. The mouse
kl gene spans about 50 kilobases and consists of five exons. The promoter region lacks a TATA-box and contains four potential binding sites for SP1. We further show that two
kl gene transcripts encoding membrane or secreted protein are generated through alternative transcriptional termination. These findings provide fundamental information for further study of the
kl gene which may regulate aging in vivo.</description><subject>Alternative Splicing</subject><subject>Alternative transcriptional termination</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Competitive polymerase chain reaction</subject><subject>Glucuronidase</subject><subject>Humans</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Mouse klotho gene</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Transcription, Genetic</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNo9UctKBDEQDKLo-vgEISfRw2h6M5kkJxHxBYIH9RwySY9Gd2bWJKP490Z38dRUV9GPKkIOgZ0Cg-bskTGoKyE1P9bqpIC5rJoNMgMlecXrRm2S2b9kh-ym9MYKVqC3ybYWXAomZsQ-5ji5PEWkY0fzK9J-nBLS98WYX0f6ggNSO3gacqL5a6Q52iG5GJYF4-BGH4YX2mPflv5KmdBFzOjpMo4Zw7BPtjq7SHiwrnvk-frq6fK2un-4ubu8uK8chzpXYi6hlda23s9RAxOIsmaMowPoujmXsnFCIxeaOwUglGutA9XWwtdgbcf3yNFqbtn7MWHKpg_J4WJRDisvGamlVDXIIjxcC6e2R2-WMfQ2fpu1J4U_X_FYrv0MGE1yofyKPkR02fgxGGDmNwTzF4L5ddhoZf5CMA3_Ac0NedU</recordid><startdate>19980306</startdate><enddate>19980306</enddate><creator>Shiraki-Iida, Takako</creator><creator>Aizawa, Hiroki</creator><creator>Matsumura, Yutaka</creator><creator>Sekine, Susumu</creator><creator>Iida, Akihiro</creator><creator>Anazawa, Hideharu</creator><creator>Nagai, Ryozo</creator><creator>Kuro-o, Makoto</creator><creator>Nabeshima, Yo-ichi</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19980306</creationdate><title>Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein</title><author>Shiraki-Iida, Takako ; Aizawa, Hiroki ; Matsumura, Yutaka ; Sekine, Susumu ; Iida, Akihiro ; Anazawa, Hideharu ; Nagai, Ryozo ; Kuro-o, Makoto ; Nabeshima, Yo-ichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c314t-5271b7aabdd2e9105ee74003ec11ff23776c59e3593c81158cbac18b45d41aaf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alternative Splicing</topic><topic>Alternative transcriptional termination</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Competitive polymerase chain reaction</topic><topic>Glucuronidase</topic><topic>Humans</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Mouse klotho gene</topic><topic>Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shiraki-Iida, Takako</creatorcontrib><creatorcontrib>Aizawa, Hiroki</creatorcontrib><creatorcontrib>Matsumura, Yutaka</creatorcontrib><creatorcontrib>Sekine, Susumu</creatorcontrib><creatorcontrib>Iida, Akihiro</creatorcontrib><creatorcontrib>Anazawa, Hideharu</creatorcontrib><creatorcontrib>Nagai, Ryozo</creatorcontrib><creatorcontrib>Kuro-o, Makoto</creatorcontrib><creatorcontrib>Nabeshima, Yo-ichi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shiraki-Iida, Takako</au><au>Aizawa, Hiroki</au><au>Matsumura, Yutaka</au><au>Sekine, Susumu</au><au>Iida, Akihiro</au><au>Anazawa, Hideharu</au><au>Nagai, Ryozo</au><au>Kuro-o, Makoto</au><au>Nabeshima, Yo-ichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1998-03-06</date><risdate>1998</risdate><volume>424</volume><issue>1</issue><spage>6</spage><epage>10</epage><pages>6-10</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We previously established a novel mouse model for human aging and identified the genetic foundation responsible for it. A defect in expression of a novel gene, termed
klotho (
kl), leads to a syndrome resembling human aging in mice. The
kl gene encodes a single-pass membrane protein whose extracellular domain carries homology to
β-glucosidases. In this report, we present the entire mouse
kl gene organization. The mouse
kl gene spans about 50 kilobases and consists of five exons. The promoter region lacks a TATA-box and contains four potential binding sites for SP1. We further show that two
kl gene transcripts encoding membrane or secreted protein are generated through alternative transcriptional termination. These findings provide fundamental information for further study of the
kl gene which may regulate aging in vivo.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9537505</pmid><doi>10.1016/S0014-5793(98)00127-6</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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source | ScienceDirect; Wiley-Blackwell Read & Publish Collection |
subjects | Alternative Splicing Alternative transcriptional termination Amino Acid Sequence Animals Base Sequence Competitive polymerase chain reaction Glucuronidase Humans Membrane Proteins - genetics Membrane Proteins - metabolism Mice Molecular Sequence Data Mouse klotho gene Polymerase Chain Reaction RNA, Messenger - metabolism Transcription, Genetic |
title | Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein |
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