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Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein

We previously established a novel mouse model for human aging and identified the genetic foundation responsible for it. A defect in expression of a novel gene, termed klotho ( kl), leads to a syndrome resembling human aging in mice. The kl gene encodes a single-pass membrane protein whose extracellu...

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Published in:FEBS letters 1998-03, Vol.424 (1), p.6-10
Main Authors: Shiraki-Iida, Takako, Aizawa, Hiroki, Matsumura, Yutaka, Sekine, Susumu, Iida, Akihiro, Anazawa, Hideharu, Nagai, Ryozo, Kuro-o, Makoto, Nabeshima, Yo-ichi
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container_issue 1
container_start_page 6
container_title FEBS letters
container_volume 424
creator Shiraki-Iida, Takako
Aizawa, Hiroki
Matsumura, Yutaka
Sekine, Susumu
Iida, Akihiro
Anazawa, Hideharu
Nagai, Ryozo
Kuro-o, Makoto
Nabeshima, Yo-ichi
description We previously established a novel mouse model for human aging and identified the genetic foundation responsible for it. A defect in expression of a novel gene, termed klotho ( kl), leads to a syndrome resembling human aging in mice. The kl gene encodes a single-pass membrane protein whose extracellular domain carries homology to β-glucosidases. In this report, we present the entire mouse kl gene organization. The mouse kl gene spans about 50 kilobases and consists of five exons. The promoter region lacks a TATA-box and contains four potential binding sites for SP1. We further show that two kl gene transcripts encoding membrane or secreted protein are generated through alternative transcriptional termination. These findings provide fundamental information for further study of the kl gene which may regulate aging in vivo.
doi_str_mv 10.1016/S0014-5793(98)00127-6
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ispartof FEBS letters, 1998-03, Vol.424 (1), p.6-10
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source ScienceDirect; Wiley-Blackwell Read & Publish Collection
subjects Alternative Splicing
Alternative transcriptional termination
Amino Acid Sequence
Animals
Base Sequence
Competitive polymerase chain reaction
Glucuronidase
Humans
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mice
Molecular Sequence Data
Mouse klotho gene
Polymerase Chain Reaction
RNA, Messenger - metabolism
Transcription, Genetic
title Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein
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