HPLC assays to simultaneously determine the angiotensin-AT1 antagonist losartan as well as its main and active metabolite EXP 3174 in biological material of humans and rats

Novel rapid and sensitive HPLC assays were developed to simultaneously determine losartan and its main active metabolite EXP 3174 in biological material of humans and rats following solid-phase or liquid-liquid extraction. The analytes were separated on a 3 microm particle-sized ULTREMEX CN column,...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1998, Vol.16 (5), p.863-873
Main Authors: SOLDNER, A, SPAHN-LANGGUTH, H, MUTSCHLER, E
Format: Article
Language:English
Subjects:
Analysis
Angiotensin I - metabolism
Angiotensin Receptor Antagonists
Animals
Antihypertensive Agents - blood
Antihypertensive Agents - pharmacokinetics
Antihypertensive Agents - urine
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
General pharmacology
Humans
Imidazoles - blood
Imidazoles - pharmacokinetics
Imidazoles - urine
Losartan - blood
Losartan - pharmacokinetics
Losartan - urine
Medical sciences
Pharmacology. Drug treatments
Rats
Receptors, Angiotensin - metabolism
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Tetrazoles - blood
Tetrazoles - pharmacokinetics
Tetrazoles - urine
Tissue Distribution
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Summary:Novel rapid and sensitive HPLC assays were developed to simultaneously determine losartan and its main active metabolite EXP 3174 in biological material of humans and rats following solid-phase or liquid-liquid extraction. The analytes were separated on a 3 microm particle-sized ULTREMEX CN column, which was preceded by a 5 microm particle-sized guard column, using UV-detection at 245 nm. The assays provided high sensitivity with limits of quantification (LoQ) of 5 ng ml(-1) for both compounds in human and rat plasma and 10 ng ml(-1) in human and rat urine, respectively. In rat blood, bile and various tissues, limits of quantifications were achieved that ranged 10-15 ng per ml and per 100 mg tissue, respectively, for both analytes.
ISSN:0731-7085
1873-264X
DOI:10.1016/s0731-7085(97)00128-3