Sulfated glycosaminoglycans in guinea pig basophils studied by means of cationic colloidal gold

Bone marrow embedding in the hydrophilic resin, Lowicryl K4M, followed by cationic colloidal gold (CCG, pH 1.0) staining was used to study the sulfated glycosaminoglycans (GAGs) and their sites of sulfation ultrastructurally in various maturational stages of both basophil granulocytes and basophil g...

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Bibliographic Details
Published in:Histochemistry and cell biology 1998-03, Vol.109 (3), p.189-194
Main Authors: Yang, D H, Tsuyama, S, Ohmori, J, Murata, F
Format: Article
Language:English
Subjects:
Animals
Basophils - chemistry
Bone marrow
Chondroitin ABC lyase
Embedding
Glycosaminoglycans
Glycosaminoglycans - analysis
Gold
Golgi apparatus
Granulocytes
Guinea Pigs
Leukocytes (basophilic)
Leukocytes (granulocytic)
Myelocytes
pH effects
Polylysine
Ultrastructure
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Summary:Bone marrow embedding in the hydrophilic resin, Lowicryl K4M, followed by cationic colloidal gold (CCG, pH 1.0) staining was used to study the sulfated glycosaminoglycans (GAGs) and their sites of sulfation ultrastructurally in various maturational stages of both basophil granulocytes and basophil granules in the guinea pig. CCG at pH 1.0 is specific for sulfated GAG staining. Basophil granulocytes and granules reacted positively to CCG with a variety of staining according to the stage of maturation. The formation of basophil granules takes place throughout the myelocyte stage. Early basophil myelocytes contain a large Golgi apparatus with active granulogenesis, while late myelocytes contain a small and less active Golgi apparatus as judged by CCG staining. All the immature granules and some of the granules with characteristic ultrastructure stained positively. However, some of the mature granules had lost their affinity for CCG upon maturation. Interestingly, strongly positive CCG staining was also observed in the trans to transmost Golgi apparatus. This indicates that sulfation of GAGs occurs in the trans to transmost Golgi apparatus in all maturational stages of basophil granulocytes. Treatment with chondroitinase ABC or heparinase I abolished the majority of CCG staining.
ISSN:0948-6143
1432-119X
DOI:10.1007/s004180050217