Effects of norepinephrine on expression of IGF-1/IGF-1R and SERCA2 in rat heart

The effects of norepinephrine on expression of cardiac genes during pathological cardiac growth and heart failure are not fully understood. Tissue insulin-like growth factor 1 (IGF-1) and its receptor (IGF-1R) play an important role in the regulation of the hyperplastic capacity of cardiac myocytes....

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Bibliographic Details
Published in:Cardiovascular research 1998, Vol.37 (1), p.202-209
Main Authors: SUN, X, NG, Y.-C
Format: Article
Language:English
Subjects:
Adrenergic alpha-Agonists - pharmacology
Animals
Biological and medical sciences
Blotting, Northern
Calcium-Transporting ATPases - genetics
Cardiovascular system
Gene Expression - drug effects
Genetic Techniques
Insulin-Like Growth Factor I - genetics
Investigative techniques, diagnostic techniques (general aspects)
Linear Models
Male
Medical sciences
Myocardium - metabolism
Norepinephrine - pharmacology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Rats
Rats, Sprague-Dawley
Receptor, IGF Type 1 - genetics
RNA, Messenger - metabolism
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Summary:The effects of norepinephrine on expression of cardiac genes during pathological cardiac growth and heart failure are not fully understood. Tissue insulin-like growth factor 1 (IGF-1) and its receptor (IGF-1R) play an important role in the regulation of the hyperplastic capacity of cardiac myocytes. Sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2), on the other hand, is important in regulating cardiac contractile function. The present study examined the effects of elevated levels of NE on expression of IGF-1/IGF-1R and SERCA2 mRNAs. Rats were infused with NE using osmotic minipumps for 3 and 6 days at a rate of 50 micrograms/kg/h and also at a higher dose (130 micrograms/kg/h) for 6 and 14 days. Levels of expression of IGF-1/IGF-1R and SERCA2 mRNAs were determined by ribonuclease protection assay and by Northern blotting, respectively. NE treatment significantly increased IGF-1 mRNA levels in both left- and right-ventricle; however, levels of IGF-1R increased in the left- but not the right-ventricle. By contrast, NE infusion at both the lower dose and the higher dose failed to alter expression of SERCA2 mRNA. Our results suggest that NE treatment differentially regulates expression of IGF-1 and IGF-1R in the ventricles of rat heart and that NE appears not to affect expression of SERCA2 mRNA.
ISSN:0008-6363
1755-3245
DOI:10.1016/S0008-6363(97)00210-1