Heteronuclear X-filter 1H PFG double-quantum experiments for the proton resonance assignment of a ligand bound to a protein

A novel X-filter experiment based on 1H PFG DQ spectroscopy is described. Excellent suppression of proton bound to 13C and 15N is achieved. Successful application of the method to a protein-ligand complex is demonstrated.

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Bibliographic Details
Published in:Journal of magnetic resonance (1997) 1998-03, Vol.131 (1), p.148-153
Main Authors: Dalvit, C, Ramage, P, Hommel, U
Format: Article
Language:English
Subjects:
Algorithms
Carbon Isotopes
Electron Spin Resonance Spectroscopy
Glucose - chemistry
Hydrogen
Image Enhancement - methods
Ligands
Lymphocyte Function-Associated Antigen-1 - chemistry
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular - methods
Phenylalanine - chemistry
Protein Binding
Protons
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Description
Summary:A novel X-filter experiment based on 1H PFG DQ spectroscopy is described. Excellent suppression of proton bound to 13C and 15N is achieved. Successful application of the method to a protein-ligand complex is demonstrated.
ISSN:1090-7807