Comparison of Heparin-sensitive Attachment of Pseudorabies Virus (PRV) and Herpes Simplex Virus Type 1 and Identification of Heparin-binding PRV Glycoproteins

• Published in: Journal of general virology 1990-05, Vol.71 (5), p.1221-1225
• Main Authors: Sawitzky, Dirk, Hampl, Hartmut, Habermehl, Karl-Otto
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cell Line; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Heparin - pharmacology; Heparitin Sulfate - metabolism; herpes simplex virus 1; Herpesvirus 1, Suid - drug effects; Herpesvirus 1, Suid - growth & development; Herpesvirus 1, Suid - metabolism; Microbiology; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Simplexvirus - drug effects; Simplexvirus - growth & development; Simplexvirus - metabolism; Viral Envelope Proteins - metabolism; Viral Plaque Assay; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-71-5-1221

Institute for Clinical and Experimental Virology, Free University Berlin, Hindenburgdamm 27, 1000 Berlin 45, F.R.G. To determine whether heparan sulphate residues on the cellular surface could serve as an attachment receptor for pseudorabies virus (PRV), the effect of heparin on PRV in plaque reduction and adsorption tests was investigated. PRV was significantly less sensitive to heparin than was herpes simplex virus type 1 (HSV-1). At concentrations of 500 µg/ml heparin the number of plaques formed by PRV was reduced to 7% of the untreated control whereas the number of plaques formed by HSV-1 was reduced to below 0.1%. Adsorption of PRV to host cells was also less sensitive to heparin treatment than was adsorption of HSV-1. Experiments concerning the binding sites of PRV showed that heparin binds to the disulphide-linked glycoprotein complex gII (PRV gB), gIII (PRV gC) and probably gV. Received 7 August 1989; accepted 3 January 1990.