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Heterologous reassortment of bunyaviruses in Aedes triseriatus mosquitoes and transovarial and oral transmission of newly evolved genotypes
1 Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523 2 Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, U.S.A. and 3 NERC Institute of Virology and Environmental Microb...
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| Published in: | Journal of general virology 1990-05, Vol.71 (5), p.1045-1050 |
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| Main Authors: | , , , , |
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| container_end_page | 1050 |
| container_issue | 5 |
| container_start_page | 1045 |
| container_title | Journal of general virology |
| container_volume | 71 |
| creator | Chandler, L.J Beaty, B.J Baldridge, G.D Bishop, D.H.L Hewlett, M.J |
| description | 1 Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523
2 Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, U.S.A.
and 3 NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, U.K.
Aedes triseriatus mosquitoes were orally infected with two different California serogroup bunyaviruses (La Crosse and snowshoe hare viruses) and high frequency reassortment occurred in these mosquitoes. Increased viral replication and subsequent gene segment reassortment was noted in the ovaries of mosquitoes that had ingested multiple blood-meals. To determine whether newly generated reassortant viruses could be transmitted transovarially to progeny mosquitoes, adult female mosquitoes were inoculated with the two temperature-sensitive (ts) parental viruses, and allowed to blood-feed and oviposit. Of 58 infected progeny mosquitoes assayed, six (10%) contained non-ts viruses, and three of these transmitted non-ts viruses to a susceptible host. Selected viruses of the non-ts phenotype, which were isolated from mosquitoes and from mice fed upon by the mosquitoes, were demonstrated to be reassortant viruses by oligonucleotide fingerprinting.
Received 27 September 1989;
accepted 6 February 1990. |
| doi_str_mv | 10.1099/0022-1317-71-5-1045 |
| format | article |
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2 Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, U.S.A.
and 3 NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, U.K.
Aedes triseriatus mosquitoes were orally infected with two different California serogroup bunyaviruses (La Crosse and snowshoe hare viruses) and high frequency reassortment occurred in these mosquitoes. Increased viral replication and subsequent gene segment reassortment was noted in the ovaries of mosquitoes that had ingested multiple blood-meals. To determine whether newly generated reassortant viruses could be transmitted transovarially to progeny mosquitoes, adult female mosquitoes were inoculated with the two temperature-sensitive (ts) parental viruses, and allowed to blood-feed and oviposit. Of 58 infected progeny mosquitoes assayed, six (10%) contained non-ts viruses, and three of these transmitted non-ts viruses to a susceptible host. Selected viruses of the non-ts phenotype, which were isolated from mosquitoes and from mice fed upon by the mosquitoes, were demonstrated to be reassortant viruses by oligonucleotide fingerprinting.
Received 27 September 1989;
accepted 6 February 1990.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-71-5-1045</identifier><identifier>PMID: 2345365</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Aedes - microbiology ; Aedes triseriatus ; Animals ; Biological and medical sciences ; Bunyaviridae ; Bunyaviridae - genetics ; Cell Line ; Culicidae ; Diptera ; disease transmission ; disease vectors ; Encephalitis Virus, California - genetics ; Encephalitis Virus, California - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; gene segment reassortment ; Mice ; Microbiology ; Oviposition ; Ovum - microbiology ; Phenotype ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; snowshoe hare virus ; Temperature ; Virology ; Virus Replication</subject><ispartof>Journal of general virology, 1990-05, Vol.71 (5), p.1045-1050</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3775-953558eec7b4442f29dd0487f3e1ad46ea5e15edc42ddb23952c1013486e43ed3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6934761$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2345365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chandler, L.J</creatorcontrib><creatorcontrib>Beaty, B.J</creatorcontrib><creatorcontrib>Baldridge, G.D</creatorcontrib><creatorcontrib>Bishop, D.H.L</creatorcontrib><creatorcontrib>Hewlett, M.J</creatorcontrib><title>Heterologous reassortment of bunyaviruses in Aedes triseriatus mosquitoes and transovarial and oral transmission of newly evolved genotypes</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523
2 Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, U.S.A.
and 3 NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, U.K.
Aedes triseriatus mosquitoes were orally infected with two different California serogroup bunyaviruses (La Crosse and snowshoe hare viruses) and high frequency reassortment occurred in these mosquitoes. Increased viral replication and subsequent gene segment reassortment was noted in the ovaries of mosquitoes that had ingested multiple blood-meals. To determine whether newly generated reassortant viruses could be transmitted transovarially to progeny mosquitoes, adult female mosquitoes were inoculated with the two temperature-sensitive (ts) parental viruses, and allowed to blood-feed and oviposit. Of 58 infected progeny mosquitoes assayed, six (10%) contained non-ts viruses, and three of these transmitted non-ts viruses to a susceptible host. Selected viruses of the non-ts phenotype, which were isolated from mosquitoes and from mice fed upon by the mosquitoes, were demonstrated to be reassortant viruses by oligonucleotide fingerprinting.
Received 27 September 1989;
accepted 6 February 1990.</description><subject>Aedes - microbiology</subject><subject>Aedes triseriatus</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bunyaviridae</subject><subject>Bunyaviridae - genetics</subject><subject>Cell Line</subject><subject>Culicidae</subject><subject>Diptera</subject><subject>disease transmission</subject><subject>disease vectors</subject><subject>Encephalitis Virus, California - genetics</subject><subject>Encephalitis Virus, California - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene segment reassortment</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Oviposition</subject><subject>Ovum - microbiology</subject><subject>Phenotype</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>snowshoe hare virus</subject><subject>Temperature</subject><subject>Virology</subject><subject>Virus Replication</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQxi0EKtvCEyBEDlXFJeD_Xh-rCihSJQ7Qs-XEk61REm_tZKt9Bl6aSXe14oZ8sDXfb8aj7yPkHaOfGLX2M6Wc10wwUxtWq5pRqV6QFZNa1Rz1l2R1Il6T81J-U8qkVOaMnHEhldBqRf7cwgQ59WmT5lJl8KWkPA0wTlXqqmYe934X81ygVHGsriHgY8qxQI5-wo4hlcc5TgnLfgwo-bGknUe1fy6kjI_n6hBLiWlcxo7w1O8r2KV-B6HawJim_RbKG_Kq832Bt8f7gtx__fLr5ra--_Ht-831Xd0KY1RtlVBqDdCaRkrJO25DoHJtOgHMB6nBK2AKQit5CA0XVvGWUSbkWoMUEMQFuTrM3eb0OEOZHO7WQt_7EdAFZywepvl_Qaa0ktwoBMUBbHMqJUPntjkOPu8do27Jyi1JuCUJZ5hTbskKu94fx8_NAOHUcwwH9cuj7kvr-w5dbGM5YdoKaTRD7OMBe4ibh6eYwaGjQ8RVmpgcpvfvjx8OaOeT8xvM0d3_5OgN5dpaabX4CxUAtxE</recordid><startdate>199005</startdate><enddate>199005</enddate><creator>Chandler, L.J</creator><creator>Beaty, B.J</creator><creator>Baldridge, G.D</creator><creator>Bishop, D.H.L</creator><creator>Hewlett, M.J</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199005</creationdate><title>Heterologous reassortment of bunyaviruses in Aedes triseriatus mosquitoes and transovarial and oral transmission of newly evolved genotypes</title><author>Chandler, L.J ; Beaty, B.J ; Baldridge, G.D ; Bishop, D.H.L ; Hewlett, M.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3775-953558eec7b4442f29dd0487f3e1ad46ea5e15edc42ddb23952c1013486e43ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Aedes - microbiology</topic><topic>Aedes triseriatus</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bunyaviridae</topic><topic>Bunyaviridae - genetics</topic><topic>Cell Line</topic><topic>Culicidae</topic><topic>Diptera</topic><topic>disease transmission</topic><topic>disease vectors</topic><topic>Encephalitis Virus, California - genetics</topic><topic>Encephalitis Virus, California - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene segment reassortment</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Oviposition</topic><topic>Ovum - microbiology</topic><topic>Phenotype</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>snowshoe hare virus</topic><topic>Temperature</topic><topic>Virology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chandler, L.J</creatorcontrib><creatorcontrib>Beaty, B.J</creatorcontrib><creatorcontrib>Baldridge, G.D</creatorcontrib><creatorcontrib>Bishop, D.H.L</creatorcontrib><creatorcontrib>Hewlett, M.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chandler, L.J</au><au>Beaty, B.J</au><au>Baldridge, G.D</au><au>Bishop, D.H.L</au><au>Hewlett, M.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterologous reassortment of bunyaviruses in Aedes triseriatus mosquitoes and transovarial and oral transmission of newly evolved genotypes</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1990-05</date><risdate>1990</risdate><volume>71</volume><issue>5</issue><spage>1045</spage><epage>1050</epage><pages>1045-1050</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>1 Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523
2 Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, U.S.A.
and 3 NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, U.K.
Aedes triseriatus mosquitoes were orally infected with two different California serogroup bunyaviruses (La Crosse and snowshoe hare viruses) and high frequency reassortment occurred in these mosquitoes. Increased viral replication and subsequent gene segment reassortment was noted in the ovaries of mosquitoes that had ingested multiple blood-meals. To determine whether newly generated reassortant viruses could be transmitted transovarially to progeny mosquitoes, adult female mosquitoes were inoculated with the two temperature-sensitive (ts) parental viruses, and allowed to blood-feed and oviposit. Of 58 infected progeny mosquitoes assayed, six (10%) contained non-ts viruses, and three of these transmitted non-ts viruses to a susceptible host. Selected viruses of the non-ts phenotype, which were isolated from mosquitoes and from mice fed upon by the mosquitoes, were demonstrated to be reassortant viruses by oligonucleotide fingerprinting.
Received 27 September 1989;
accepted 6 February 1990.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>2345365</pmid><doi>10.1099/0022-1317-71-5-1045</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1990-05, Vol.71 (5), p.1045-1050 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79797162 |
| source | Freely Accessible Science Journals |
| subjects | Aedes - microbiology Aedes triseriatus Animals Biological and medical sciences Bunyaviridae Bunyaviridae - genetics Cell Line Culicidae Diptera disease transmission disease vectors Encephalitis Virus, California - genetics Encephalitis Virus, California - physiology Female Fundamental and applied biological sciences. Psychology gene segment reassortment Mice Microbiology Oviposition Ovum - microbiology Phenotype Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains snowshoe hare virus Temperature Virology Virus Replication |
| title | Heterologous reassortment of bunyaviruses in Aedes triseriatus mosquitoes and transovarial and oral transmission of newly evolved genotypes |
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