Metastasis of Theileria annulata macroschizont-infected cells in scid mice is mediated by matrix metalloproteinases

Theileria annulata (Ta)-infected leucocytes are able to disseminate in scid mice. The dose of virulent parasites of the Ta-Ode line required to achieve quantifiable dissemination was found to be 2×10 cells given i.p. Dissemination was higher on day 11 post-inoculation than on day 18. The attenuated...

Full description

Saved in:
Bibliographic Details
Published in:Parasitology 1998-03, Vol.116 (3), p.223-228, Article S0031182097002151
Main Authors: SOMERVILLE, R. P. T., ADAMSON, R. E., BROWN, C. G. D., HALL, F. R.
Format: Article
Language:English
Subjects:
Animals
attenuation
BB94
Biological and medical sciences
Cattle
Cell Count
Experimental protozoal diseases and models
Female
Infectious diseases
Kidney - parasitology
Leukocytes - parasitology
Liver - parasitology
Lung - parasitology
macroschizont-infected cells
macroschizonts
matrix metalloproteinase
Medical sciences
Mesentery - parasitology
Metalloendopeptidases - antagonists & inhibitors
Metalloendopeptidases - metabolism
metastasis
Mice
Mice, SCID - parasitology
Parasitic diseases
Phenylalanine - analogs & derivatives
Phenylalanine - pharmacology
Protease Inhibitors - pharmacology
Protozoal diseases
scid mice
Spleen - parasitology
Theileria annulata
Theileria annulata - growth & development
Theileria annulata - pathogenicity
Theileria annulata - physiology
Theileriasis - enzymology
Theileriasis - parasitology
Theiliera annulata
Thiophenes - pharmacology
Time Factors
Virulence
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Theileria annulata (Ta)-infected leucocytes are able to disseminate in scid mice. The dose of virulent parasites of the Ta-Ode line required to achieve quantifiable dissemination was found to be 2×10 cells given i.p. Dissemination was higher on day 11 post-inoculation than on day 18. The attenuated Ta-Ode cells were found to disseminate very poorly compared to their virulent progenitors, which correlates with a marked reduction in matrix metalloproteinase (MMP) expression. A daily i.p. injection of mice with BB94, a synthetic inhibitor of MMPs, almost completely ablated dissemination compared to controls. This provides strong evidence that metastasis of Theileria annulata macroschizont-infected host cells is mediated by host MMPs induced by the parasite. This has important implications for explaining a number of pathological features of tropical theileriosis in cattle.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182097002151