Validation of an LC-MS assay for the quantification of the enantiomers of Org 4428 in human plasma

A sensitive and selective liquid chromatographic mass spectrometric assay has been validated for the quantification of Org 4428 enantiomers in human plasma. The assay employs n-hexane extraction from alkalinized plasma, separation on a narrow-bore enantioselective normal phase Chiralpak AD column an...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1998-02, Vol.16 (6), p.981-989
Main Authors: Paanakker, J.E, de Jong, J, Thio, J.M.S.L, van Hal, H.J.M
Format: Article
Language:English
Subjects:
Adrenergic Uptake Inhibitors - blood
Adrenergic Uptake Inhibitors - chemistry
Analysis
Antidepressant
Biological and medical sciences
Calibration
Chromatography, Liquid
Freezing
General pharmacology
Human plasma
Humans
Indicators and Reagents
LC-MS
Mass Spectrometry
Medical sciences
Org 4428 enantiomers
Pharmacology. Drug treatments
Pyridines - blood
Pyridines - chemistry
Reproducibility of Results
Stereoisomerism
Validation
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Summary:A sensitive and selective liquid chromatographic mass spectrometric assay has been validated for the quantification of Org 4428 enantiomers in human plasma. The assay employs n-hexane extraction from alkalinized plasma, separation on a narrow-bore enantioselective normal phase Chiralpak AD column and APCI MS-MS detection. The lower limit of quantification is 0.5 ng ml −1 plasma for the individual enantiomers.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(97)00113-1