A Periarteriolar Lymphoid Sheath-Associated B Cell Focus Response Is Not Observed During the Development of the Anti-Arsonate Germinal Center Reaction

The behavior of p-azophenylarsonate (Ars)-specific B cell clones during the primary T cell-dependent splenic response of A/J mice was investigated using an immunohistochemical approach. The earliest Ars-specific B cells were observed as isolated cells in the red pulp by day 3 after immunization with...

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Bibliographic Details
Published in:The Journal of immunology (1950) 1998-01, Vol.160 (2), p.728-733
Main Authors: Vora, Kalpit A, Tumas-Brundage, Kathleen M, Manser, Tim
Format: Article
Language:English
Subjects:
Animals
Arterioles
B-Lymphocytes - cytology
B-Lymphocytes - immunology
Cell Differentiation - immunology
Chickens
Epitopes - immunology
gamma-Globulins - immunology
Germinal Center - cytology
Germinal Center - immunology
Lymphoid Tissue - blood supply
Lymphoid Tissue - cytology
Lymphoid Tissue - immunology
Mice
Mice, Inbred A
Nitrophenols - immunology
p-Azobenzenearsonate - immunology
Phenylacetates
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Summary:The behavior of p-azophenylarsonate (Ars)-specific B cell clones during the primary T cell-dependent splenic response of A/J mice was investigated using an immunohistochemical approach. The earliest Ars-specific B cells were observed as isolated cells in the red pulp by day 3 after immunization with Ars-keyhole limpet hemocyanin, (KLH) and at day 6, large clusters of Ars-specific B cells were first detected in germinal centers, which continued to be observed for an additional 8 to 15 days. Surprisingly, no Ars-specific B cell foci were observed in or near the CD4 T cell-rich periarteriolar lymphoid sheath (PALS) during the entire primary response. Nevertheless, A/J mice immunized with (4-hydroxy-3-nitrophenyl)acetyl-chicken gamma globulin (NP-CGG) or Ars-CGG mounted robust splenic (4-hydroxy-3-nitrophenyl)acetyl or CGG-specific PALS-associated focus reactions, respectively. In contrast, no Ars-specific PALS B cell foci were detected in A/J mice immunized with Ars-CGG. These data add to a growing body of evidence indicating that B cell proliferation and differentiation in CD4 T cell-rich microenvironments are not prerequisites for the GC reaction. Taken together with previous results obtained using other model Ags, the data suggest that the specificity of the B cell Ag receptor may strongly influence the lymphoid microenvironment in which a B cell clone first undergoes Ag-driven clonal expansion and differentiation.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.160.2.728