Determination of free and total carnitine with a random-access chemistry analyzer

Carnitine deficiency presents as a major problem in fatty acid oxidation. The use of a plasma carnitine assay can rapidly help to describe this deficiency. The method we describe here requires two simple steps of sample preparation, followed by automated analysis with the Beckman Synchron CX4 random...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 1998-04, Vol.44 (4), p.810-816
Main Authors: Wan, Lijun, Hubbard, Richard W
Format: Article
Language:English
Subjects:
Adolescent
Adult
Analytical, structural and metabolic biochemistry
Autoanalysis - instrumentation
Biological and medical sciences
Blood Proteins - metabolism
Carnitine - blood
Carnitine - metabolism
Child
Child, Preschool
Fundamental and applied biological sciences. Psychology
Humans
Infant
Infant, Newborn
Intermediary metabolites. Miscellaneous
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
Other biological molecules
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Protein Binding
Reference Values
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
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Summary:Carnitine deficiency presents as a major problem in fatty acid oxidation. The use of a plasma carnitine assay can rapidly help to describe this deficiency. The method we describe here requires two simple steps of sample preparation, followed by automated analysis with the Beckman Synchron CX4 random-access chemistry analyzer. The goal of this method development was to reduce the cost of analysis and to allow a greater number of laboratories to perform this assay on demand within 1 h for both free and total carnitine. The method has a linearity of 0-150 micromol/L and a detection limit of 5 micromol/L. The inter- and intraday CVs are
ISSN:0009-9147
1530-8561