A prospective, randomized, double-blind study for the evaluation of assisted hatching in patients with advanced maternal age

The objective of this study was to determine if assisted hatching improved the rates of implantation, clinical pregnancy and ongoing pregnancy for in-vitro fertilization (IVF) patients aged > or =36 years. On the day of oocyte aspiration, consenting patients were randomized according to whether a...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1998-02, Vol.13 (2), p.409-413
Main Authors: LANZENDORF, S. E, NEHCHIRI, F, MAYER, J. F, OEHNINGER, S, MUASHER, S. J
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Birth control
Blastocyst - cytology
Culture Media
Double-Blind Method
Embryo Transfer
Female
Fertilization in Vitro - methods
Gynecology. Andrology. Obstetrics
Humans
Infertility - therapy
Isotonic Solutions
Male
Maternal Age
Medical sciences
Pregnancy
Prospective Studies
Sterility. Assisted procreation
Zona Pellucida - ultrastructure
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Summary:The objective of this study was to determine if assisted hatching improved the rates of implantation, clinical pregnancy and ongoing pregnancy for in-vitro fertilization (IVF) patients aged > or =36 years. On the day of oocyte aspiration, consenting patients were randomized according to whether all embryos underwent the hatching procedure (hatched; n = 41) or all embryos remained unhatched (controls; n = 48). Patients in both groups were treated with methylprednisolone and doxycycline starting on the day of oocyte retrieval and continuing for 4 days. The hatching procedure was performed approximately 55 h after insemination on all potential embryos for transfer and employed the release of acidified acid Tyrode's medium against the zona pellucida to create an opening approximately 20 microm in diameter. No significant differences were noted in the mean age, number of oocytes aspirated and number of embryos transferred between the hatched and control groups. In addition, no significant differences were observed in the rates of implantation (11.1 versus 11.3%), clinical pregnancy (39.0 versus 41.7%) and ongoing pregnancy (29.3 versus 35.4%) between the hatched and control groups respectively. These results suggest that assisted hatching may have no significant impact on IVF success rates in the patient population studied.
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/13.2.409