Rapid stress-induced elevations in corticotropin-releasing hormone mRNA in rat central amygdala nucleus and hypothalamic paraventricular nucleus: An in situ hybridization analysis

High densities of nerve cells containing corticotropin-releasing hormone (CRH) are located in the central nucleus of the amygdala (CeA) and paraventricular nucleus (PVN) of the hypothalamus. These brain regions play an important role in activating autonomic, behavioral, and endocrine responses to st...

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Bibliographic Details
Published in:Brain research 1998-03, Vol.788 (1), p.305-310
Main Authors: Hsu, David T., Chen, Feng-Ling, Takahashi, Lorey K., Kalin, Ned H.
Format: Article
Language:English
Subjects:
Amygdala
Amygdala - cytology
Amygdala - metabolism
Animals
Biological and medical sciences
Central amygdala nucleus
Corticotropin-releasing hormone
Corticotropin-Releasing Hormone - genetics
Fundamental and applied biological sciences. Psychology
Hormones and neuropeptides. Regulation
Hypothalamus. Hypophysis. Epiphysis. Urophysis
In Situ Hybridization
Male
mRNA
Neurons - metabolism
Neuropeptide
Paraventricular Hypothalamic Nucleus - cytology
Paraventricular Hypothalamic Nucleus - metabolism
Paraventricular nucleus
Rats
Rats, Sprague-Dawley
RNA, Messenger - biosynthesis
Stress
Stress, Physiological - metabolism
Time Factors
Vertebrates: endocrinology
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Summary:High densities of nerve cells containing corticotropin-releasing hormone (CRH) are located in the central nucleus of the amygdala (CeA) and paraventricular nucleus (PVN) of the hypothalamus. These brain regions play an important role in activating autonomic, behavioral, and endocrine responses to stress. This study was conducted to provide needed information concerning the acute effects of stress on CeA and PVN CRH mRNA expression. Rats were exposed to restraint stress for 1 h and brains collected after a 1-h post-stress interval. CRH mRNA expression occurring in the CeA and PVN was examined using in situ hybridization techniques. Densitometric analysis revealed that acute restraint stress produced significant increases in CRH mRNA levels in the PVN and in the rostral CeA region. In addition, the area in the rostral CeA encompassing high CRH mRNA signals increased significantly after stress. Results provide clear evidence that CRH neurons in the CeA and PVN exhibit rapid increases in CRH mRNA expression after exposure to stress.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(98)00032-8