Conversion of mannose to fructose by immobilized mannose isomerase from Pseudomonas cepacia

The enzyme mannose isomerase (EC 5.3.1.7) catalyzes the isomerization of D-mannose and D-fructose. The conversion of mannose to fructose is the first step in the principal pathway of mannose dissimilation by Pseudomonas cepacia. This enzyme is induced during growth on medium containing mannose to le...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1990, Vol.24-25, p.171-182
Main Authors: Allenza, P, Morrell, M J, Detroy, R W
Format: Article
Language:English
Subjects:
Aldose-Ketose Isomerases
Carbohydrate Epimerases - isolation & purification
Carbohydrate Epimerases - metabolism
Enzymes, Immobilized - metabolism
Fructose - biosynthesis
Kinetics
Mannose - metabolism
Pseudomonas - enzymology
Pseudomonas cepacia
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Summary:The enzyme mannose isomerase (EC 5.3.1.7) catalyzes the isomerization of D-mannose and D-fructose. The conversion of mannose to fructose is the first step in the principal pathway of mannose dissimilation by Pseudomonas cepacia. This enzyme is induced during growth on medium containing mannose to levels three- to four-fold higher than observed during growth on glucose or citrate. Mannose isomerase was purified from extracts of mannose-grown P. cepacia and was efficiently immobilized onto a porous, noncompressible, ceramic support. The performance of the immobilized enzyme, compared with the soluble enzyme, was evaluated under a variety of operating conditions to examine its potential for use in a process for the production of fructose syrups containing a higher proportion of fructose than is currently possible using glucose isomerase.
ISSN:0273-2289
DOI:10.1007/BF02920243