Multiple factors including subgenomic RNAs and reduced viral protein expression are associated with a persistent infection by porcine rubulavirus (LPMV)

The synthesis of virus specific RNA and the expression of viral proteins in PK-15 cells persistently infected with the porcine rubulavirus LPMV have been studied at two different cell-passages following establishment of persistency (passages 25 and 65). Protein analysis of persistently infected cell...

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Bibliographic Details
Published in:Archives of virology 1998-01, Vol.143 (3), p.425-439
Main Authors: HJERTNER, B, WIMAN, A.-C, SVENDA, M, BERG, M, MORENO-LOPEZ, J, LINNE, T
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line
Fundamental and applied biological sciences. Psychology
Herpes viruses
Microbiology
Proteins
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
RNA, Messenger
RNA, Viral
Rubulavirus - genetics
Rubulavirus - metabolism
Rubulavirus - physiology
Swine
Viral Proteins - biosynthesis
Virology
Virus Latency
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Summary:The synthesis of virus specific RNA and the expression of viral proteins in PK-15 cells persistently infected with the porcine rubulavirus LPMV have been studied at two different cell-passages following establishment of persistency (passages 25 and 65). Protein analysis of persistently infected cells and the virus particles released from these failed to demonstrate the presence of the polymerase (L) protein. A decrease in the amount of the phospho- (P) protein was also noted. The genome and mRNAs, both mono- and bicistronic, could readily be identified in the persistently infected cells with the exception of the L mRNA. By analysis of transcription gradients generated using the NIH Image analysis software, as well as analysis of the editing frequency, it was concluded that the changes in viral protein levels in persistently infected cells could be associated with a reduction in the amount of L mRNA and a shift in editing of the P gene. In addition, several large subgenomic RNAs of both the internally deleted and copy-back type were found in the persistently infected cells. The relevance of these findings to the persistent state is discussed.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050050300