Type Specific and Genotype Cross Reactive B Epitopes of the L1 Protein of HPV16 Defined by a Panel of Monoclonal Antibodies

Mouse monoclonal antibodies (mAbs) were raised against the major capsid protein, L1, of human papillomavirus type 16 (HPV16), produced inEscherichia coliwith the expression plasmid pTrcL1. Epitope specificity could be assigned to 11 of these 12 antibodies using a series of linear peptides and fusion...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1998-04, Vol.243 (2), p.275-282
Main Authors: Kulski, Jerzy K., Sadleir, John W., Kelsall, Stephen R., Cicchini, Maria S., Shellam, Geoffrey, Peng, Shi Wen, Qi, Ying Mei, Galloway, Denise A., Zhou, Jian, Frazer, Ian H.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Capsid Proteins
Cattle
Cell Line
Cercopithecus aethiops
Cross Reactions
Epitopes, B-Lymphocyte - chemistry
Epitopes, B-Lymphocyte - genetics
Epitopes, B-Lymphocyte - immunology
Genotype
Humans
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Oncogene Proteins, Viral - chemistry
Oncogene Proteins, Viral - genetics
Oncogene Proteins, Viral - immunology
Papillomaviridae - chemistry
Papillomaviridae - genetics
Papillomaviridae - immunology
Recombinant Fusion Proteins - immunology
Species Specificity
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Description
Summary:Mouse monoclonal antibodies (mAbs) were raised against the major capsid protein, L1, of human papillomavirus type 16 (HPV16), produced inEscherichia coliwith the expression plasmid pTrcL1. Epitope specificity could be assigned to 11 of these 12 antibodies using a series of linear peptides and fusion proteins from HPV16. One mAb (MC53) recognized a novel linear epitope that appears to be unique to the HPV16 genotype. A further 11 mAbs were characterized as recognizing novel and previously defined linear and conformational epitopes shared among more than one HPV genotype. The apparently genotype specific mAb could be useful for the development of diagnostic tests for vegetative virus infection in clinical specimens.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1997.9011