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Type Specific and Genotype Cross Reactive B Epitopes of the L1 Protein of HPV16 Defined by a Panel of Monoclonal Antibodies
Mouse monoclonal antibodies (mAbs) were raised against the major capsid protein, L1, of human papillomavirus type 16 (HPV16), produced inEscherichia coliwith the expression plasmid pTrcL1. Epitope specificity could be assigned to 11 of these 12 antibodies using a series of linear peptides and fusion...
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Published in: | Virology (New York, N.Y.) N.Y.), 1998-04, Vol.243 (2), p.275-282 |
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container_end_page | 282 |
container_issue | 2 |
container_start_page | 275 |
container_title | Virology (New York, N.Y.) |
container_volume | 243 |
creator | Kulski, Jerzy K. Sadleir, John W. Kelsall, Stephen R. Cicchini, Maria S. Shellam, Geoffrey Peng, Shi Wen Qi, Ying Mei Galloway, Denise A. Zhou, Jian Frazer, Ian H. |
description | Mouse monoclonal antibodies (mAbs) were raised against the major capsid protein, L1, of human papillomavirus type 16 (HPV16), produced inEscherichia coliwith the expression plasmid pTrcL1. Epitope specificity could be assigned to 11 of these 12 antibodies using a series of linear peptides and fusion proteins from HPV16. One mAb (MC53) recognized a novel linear epitope that appears to be unique to the HPV16 genotype. A further 11 mAbs were characterized as recognizing novel and previously defined linear and conformational epitopes shared among more than one HPV genotype. The apparently genotype specific mAb could be useful for the development of diagnostic tests for vegetative virus infection in clinical specimens. |
doi_str_mv | 10.1006/viro.1997.9011 |
format | article |
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Epitope specificity could be assigned to 11 of these 12 antibodies using a series of linear peptides and fusion proteins from HPV16. One mAb (MC53) recognized a novel linear epitope that appears to be unique to the HPV16 genotype. A further 11 mAbs were characterized as recognizing novel and previously defined linear and conformational epitopes shared among more than one HPV genotype. The apparently genotype specific mAb could be useful for the development of diagnostic tests for vegetative virus infection in clinical specimens.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9568027</pmid><doi>10.1006/viro.1997.9011</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Capsid Proteins Cattle Cell Line Cercopithecus aethiops Cross Reactions Epitopes, B-Lymphocyte - chemistry Epitopes, B-Lymphocyte - genetics Epitopes, B-Lymphocyte - immunology Genotype Humans Mice Mice, Inbred BALB C Molecular Sequence Data Oncogene Proteins, Viral - chemistry Oncogene Proteins, Viral - genetics Oncogene Proteins, Viral - immunology Papillomaviridae - chemistry Papillomaviridae - genetics Papillomaviridae - immunology Recombinant Fusion Proteins - immunology Species Specificity |
title | Type Specific and Genotype Cross Reactive B Epitopes of the L1 Protein of HPV16 Defined by a Panel of Monoclonal Antibodies |
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