Establishment and characterization of continuous cell line MTC-SK derived from a human medullary thyroid carcinoma

Tumor cells of a human medullary thyroid carcinoma were isolated and propagated in tissue culture. Several cell lines with different morphology developed from the primary culture, among others a fibroblast-like growing cell line (MTC-F) and a cell line growing as a suspension of single cells and sph...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1990-07, Vol.50 (13), p.4160-4166
Main Authors: PFRAGNER, R, HÖFLER, H, BEHMEL, A, INGOLIC, E, WALSER, V
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calcitonin - analysis
Calcitonin Gene-Related Peptide - analysis
Carcinoma - analysis
Carcinoma - genetics
Carcinoma - pathology
Carcinoma - ultrastructure
Chromosome Aberrations
Female
Gene Rearrangement
General aspects (metabolism, cell proliferation, established cell line...)
Humans
Karyotyping
Medical sciences
Middle Aged
Somatostatin - analysis
Thyroid Neoplasms - analysis
Thyroid Neoplasms - genetics
Thyroid Neoplasms - pathology
Thyroid Neoplasms - ultrastructure
Tumor cell
Tumor Cells, Cultured - pathology
Tumors
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Summary:Tumor cells of a human medullary thyroid carcinoma were isolated and propagated in tissue culture. Several cell lines with different morphology developed from the primary culture, among others a fibroblast-like growing cell line (MTC-F) and a cell line growing as a suspension of single cells and spherical cell clusters (MTC-SK). The MTC-SK cell line was serially propagated for 90 passages, over 3 years. When examined at different times throughout the in vitro period, MTC-SK exhibited properties characteristic of medullary thyroid carcinomas: the cells maintained their epithelioid morphology; endocrine granules were demonstrated in the cytoplasm by electron microscopy; in situ hybridization confirmed the production of calcitonin- and bombesin-mRNA (gastrin releasing peptide); the cells revealed positive immunoreactivity with antibodies to calcitonin, calcitonin gene-related peptide, and bombesin. The in vitro properties of the MTC-SK cells corresponded to the results obtained from the tissue of origin. Cytogenetic studies of the MTC-F cell line revealed a supernumerary metacentric chromosome (20?). In the MTC-SK cell line the predominant findings were terminal chromosomal rearrangements most frequently concerning chromosome 11p, i.e., the locus of the calcitonin and calcitonin gene-related peptide genes and the H-ras oncogene, and a characteristic instability of the centromeric region of chromosome 16 and somatic pairing of the homologous chromosomes 16.
ISSN:0008-5472
1538-7445