Rapid heterozygote detection in Glanzmann's thrombasthenia

To assess the potential of using flow cytometric analysis of platelet glycoprotein IIb in the detection of heterozygotes in Glanzmann's thrombasthenia (GT) we compared the fluorescence intensity of anti‐CD41‐labelled platelets of obligatory heterozygotes with that of normal subjects. This study...

Full description

Saved in:
Bibliographic Details
Published in:British journal of haematology 1998-04, Vol.101 (1), p.66-69
Main Authors: SHARP, W. J, KHANDURI, U. D, CHRISTIE, B. S
Format: Article
Language:English
Subjects:
Biological and medical sciences
CD41
Cell Size
Female
Flow Cytometry
Fluorescent Antibody Technique
Genetic Carrier Screening - methods
Hematology
Heterozygote
Humans
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
MPV
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Platelet Glycoprotein GPIIb-IIIa Complex - analysis
thrombasthenia
Thrombasthenia - genetics
whole blood lysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To assess the potential of using flow cytometric analysis of platelet glycoprotein IIb in the detection of heterozygotes in Glanzmann's thrombasthenia (GT) we compared the fluorescence intensity of anti‐CD41‐labelled platelets of obligatory heterozygotes with that of normal subjects. This study showed that normal subjects had relative fluorescences occasionally overlapping with heterozygotes. Use of an independent factor, the mean platelet volume, as a correction factor for platelet size differences eliminated this overlap. This study indicates that it is possible to confidently and rapidly predict the carrier status of family members of GT sufferers, both type I and type II, by flow cytometric means.
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.1998.00655.x