Ultracytochemistry of Glycoconjugates in Rat Brunner's Gland with Labeled Lectins

Mucous glycoproteins of rat Brunner's gland were examined ultracytochemically to elucidate the intracellular localization and sites of glycosylation in relation to the functional polarity of the cell organellae using a combination of hydrophilic resin embedment and postembedding staining with l...

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Bibliographic Details
Published in:Journal of electron microscopy 1990-02, Vol.39 (1), p.26-32
Main Authors: Yotsumoto, Shinichi, Tsuyama, Shinichiro, Tashiro, Masaaki, Murata, Fusayoshi
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Brunner Glands - metabolism
Brunner Glands - ultrastructure
Brunner's gland
Dolichos biflorus agglutinin
Duodenum - ultrastructure
Female
Fundamental and applied biological sciences. Psychology
Glycoconjugates - metabolism
Glycoproteins
Helix pomatia agglutinin
Histocytochemistry - methods
Lectins - metabolism
Male
Microscopy, Electron - methods
Proteins
Rats
Recinus communis agglutinin I
Ulex europaeus agglutinin I
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Summary:Mucous glycoproteins of rat Brunner's gland were examined ultracytochemically to elucidate the intracellular localization and sites of glycosylation in relation to the functional polarity of the cell organellae using a combination of hydrophilic resin embedment and postembedding staining with labeled lectins. A few cis cisternae were stained with HPA (Helix pomatia agglutinin, specific for terminal α-N-acetylgalactosamine) and DBA (Dolichos biflorus aggulutinin, specific for terminal α-N-acetylgalactosamine). Trans cisternae as well as cisternae following secretory granules were stained with HPA and RCA-I (Recinus communis agglutinin I, specific for terminal β-galactose). UEA-I (Ulex europaeus agglutinin I, specific for terminal α-L-fucose) seemed to bind to transmost cisternae rather than trans cisternae. HPA bound to the whole cisternae, but to the cis or trans cisternae depending on a cell type. Apical mucous granules were stained with UEA-I, RCA-I, and HPA, but not with DBA. DBA seemed to be a good marker of the cis side of the Golgi apparatus and cell membrane. The glycosylation of mucous glycoprotein in rat Brunner's gland was partly clarified electron microscopically.
ISSN:0022-0744
1477-9986
1477-9986
DOI:10.1093/oxfordjournals.jmicro.a050770